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Literature summary extracted from

  • Behrens, C.; Biela, I.; Petiot-Becard, S.; Botzanowski, T.; Cianferani, S.; Sager, C.P.; Klebe, G.; Heine, A.; Reuter, K.
    Homodimer architecture of QTRT2, the noncatalytic subunit of the eukaryotic tRNA-guanine transglycosylase (2018), Biochemistry, 57, 3953-3965 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.64 overexpression of QTRT1 and QTRT2 genes in Escherichia coli BL21 CodonPlus(DE3) Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.64 vapor diffusion technique at 18°C, crystal structure of the noncatalytic subunit (QTRT2) Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.64 0.0006
-
 queuine recombinant enzyme whose subunits are produced separately and combined in a molar ratio of 1:1 for kinetic measurements, pH and temperature not specified in the publication Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.64 Mus musculus Q9JMA2 AND B8ZXI1 Q9JMA2: catalytic subunit 1 (QTRT1), B8ZXI1: accessory subunit 2 (QTRT2)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.64
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.64 guanine34 in tRNA(Tyr) + queuine
-
 Mus musculus queuine34 in tRNA(Tyr) + guanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.64 homodimer 1 * 44234 (catalytic subunit QTRT1) + 1 * 47161 (noncatalytic subunit QTRT2), noncovalent mass spectrometry Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.64 QTRT1 gene name, catalytic subunit 1 Mus musculus
2.4.2.64 QTRT2 gene name, accessory subunit 2 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.64 0.003
-
 queuine recombinant enzyme whose subunits are produced separately and combined in a molar ratio of 1:1 for kinetic measurements, pH and temperature not specified in the publication Mus musculus