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Literature summary extracted from
- Discovery of a kojibiose phosphorylase in Escherichia coli K-12 (2018), Biochemistry, 57, 2857-2867 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.230 | - |
Escherichia coli |
| 2.4.1.352 | - |
Escherichia coli |
| 5.4.2.6 | - |
Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.6 | beta-D-glucose 1-phosphate | substrate inhibition is observed at concentrations of beta-D-glucose-1-phosphate greater than 0.2 mM | Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.230 | 1.05 | - |
2-alpha-D-glucosyl-D-glucose | pH 7.5, 30°C | Escherichia coli | |
| 2.4.1.352 | 0.069 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | pH 7.5, 30°C | Escherichia coli | |
| 2.4.1.352 | 15.2 | - |
phosphate | pH 7.5, 30°C | Escherichia coli | |
| 5.4.2.6 | 0.018 | - |
beta-D-glucose 1-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 5.4.2.6 | 0.09 | - |
beta-D-mannose-1-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 5.4.2.6 | 0.47 | - |
beta-D-galactose-1-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 5.4.2.6 | 1.19 | - |
beta-D-glucose 6-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 5.4.2.6 | 10.5 | - |
beta-D-allose-1-phosphate | pH 8.0, 30°C | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.6 | Mg2+ | activation constant is 0.135 mM. In the absence of added Mg2+, the observed catalytic activity is less than 2% of the turnover observed in the presence of 2.0 mM Mg2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.6 | beta-D-Glucose 1-phosphate | Escherichia coli | - |
beta-D-Glucose 6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-Glucose 1-phosphate | Escherichia coli MG1655 | - |
beta-D-Glucose 6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-glucose 6-phosphate | Escherichia coli | - |
beta-D-glucose 1-phosphate | - |
r | |
| 5.4.2.6 | beta-D-glucose 6-phosphate | Escherichia coli MG1655 | - |
beta-D-glucose 1-phosphate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.230 | Escherichia coli | P77154 | - |
- |
| 2.4.1.230 | Escherichia coli MG1655 | P77154 | - |
- |
| 2.4.1.352 | Escherichia coli | P76041 | - |
- |
| 2.4.1.352 | Escherichia coli MG1655 | P76041 | - |
- |
| 5.4.2.6 | Escherichia coli | P77366 | - |
- |
| 5.4.2.6 | Escherichia coli MG1655 | P77366 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.230 | - |
Escherichia coli |
| 2.4.1.352 | - |
Escherichia coli |
| 5.4.2.6 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.230 | 2-alpha-D-glucosyl-D-glucose + phosphate | - |
Escherichia coli | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
| 2.4.1.230 | 2-alpha-D-glucosyl-D-glucose + phosphate | - |
Escherichia coli MG1655 | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
| 2.4.1.230 | beta-D-glucose-1-phosphate + 1,5-anhydro-D-glucitol | - |
Escherichia coli | ? + phosphate | - |
? | |
| 2.4.1.230 | beta-D-glucose-1-phosphate + 1,5-anhydro-D-glucitol | - |
Escherichia coli MG1655 | ? + phosphate | - |
? | |
| 2.4.1.230 | D-sorbitol + 1,5-anhydro-D-glucitol | - |
Escherichia coli | ? + phosphate | - |
? | |
| 2.4.1.230 | D-sorbitol + 1,5-anhydro-D-glucitol | - |
Escherichia coli MG1655 | ? + phosphate | - |
? | |
| 2.4.1.230 | L-iditol + 1,5-anhydro-D-glucitol | - |
Escherichia coli | ? + phosphate | - |
? | |
| 2.4.1.230 | L-iditol + 1,5-anhydro-D-glucitol | - |
Escherichia coli MG1655 | ? + phosphate | - |
? | |
| 2.4.1.230 | L-sorbose + 1,5-anhydro-D-glucitol | - |
Escherichia coli | ? + phosphate | - |
? | |
| 2.4.1.230 | L-sorbose + 1,5-anhydro-D-glucitol | - |
Escherichia coli MG1655 | ? + phosphate | - |
? | |
| 2.4.1.352 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
Escherichia coli | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
| 2.4.1.352 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
Escherichia coli MG1655 | alpha-D-glucopyranose 1-phosphate + D-glycerate | - |
r | |
| 2.4.1.352 | alpha-D-glucopyranose 1-phosphate + D-glycerate | less than 1% of this activity is detected in the presence of alpha-D-galactose-1-P, alpha-D-mannose-1-P, or beta-D-glucose-1-P. Less than 5% of this activity is observed with either L-glycerate, D-ribonate, D-allonate, or glycerol | Escherichia coli | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r | |
| 2.4.1.352 | alpha-D-glucopyranose 1-phosphate + D-glycerate | less than 1% of this activity is detected in the presence of alpha-D-galactose-1-P, alpha-D-mannose-1-P, or beta-D-glucose-1-P. Less than 5% of this activity is observed with either L-glycerate, D-ribonate, D-allonate, or glycerol | Escherichia coli MG1655 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
r | |
| 5.4.2.6 | beta-D-allose-1-phosphate | - |
Escherichia coli | beta-D-allose-6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-allose-1-phosphate | - |
Escherichia coli MG1655 | beta-D-allose-6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-allose-6-phosphate | - |
Escherichia coli | beta-D-allose-1-phosphate | - |
r | |
| 5.4.2.6 | beta-D-allose-6-phosphate | - |
Escherichia coli MG1655 | beta-D-allose-1-phosphate | - |
r | |
| 5.4.2.6 | beta-D-galactose-1-phosphate | - |
Escherichia coli | beta-D-galactose-6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-galactose-6-phosphate | - |
Escherichia coli | beta-D-galactose-1-phosphate | - |
r | |
| 5.4.2.6 | beta-D-Glucose 1-phosphate | - |
Escherichia coli | beta-D-Glucose 6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-Glucose 1-phosphate | the enzyme exhibits less than 1% of the catalytic activity (relative to beta-D-glucose-1-phosphate) with alpha-D-glucose-1-phosphate | Escherichia coli | beta-D-Glucose 6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-Glucose 1-phosphate | - |
Escherichia coli MG1655 | beta-D-Glucose 6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-Glucose 1-phosphate | the enzyme exhibits less than 1% of the catalytic activity (relative to beta-D-glucose-1-phosphate) with alpha-D-glucose-1-phosphate | Escherichia coli MG1655 | beta-D-Glucose 6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-glucose 6-phosphate | - |
Escherichia coli | beta-D-glucose 1-phosphate | - |
r | |
| 5.4.2.6 | beta-D-glucose 6-phosphate | - |
Escherichia coli MG1655 | beta-D-glucose 1-phosphate | - |
r | |
| 5.4.2.6 | beta-D-mannose-1-phosphate | - |
Escherichia coli | beta-D-mannose-6-phosphate | - |
r | |
| 5.4.2.6 | beta-D-mannose-6-phosphate | - |
Escherichia coli | beta-D-mannose-1-phosphate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.230 | YcjT | - |
Escherichia coli |
| 2.4.1.352 | YcjM | - |
Escherichia coli |
| 5.4.2.6 | YcjU | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.230 | 30 | - |
assay at | Escherichia coli |
| 2.4.1.352 | 30 | - |
assay at | Escherichia coli |
| 5.4.2.6 | 30 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.230 | 1.1 | - |
2-alpha-D-glucosyl-D-glucose | pH 7.5, 30°C | Escherichia coli | |
| 2.4.1.352 | 2.1 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | pH 7.5, 30°C | Escherichia coli | |
| 2.4.1.352 | 2.4 | - |
phosphate | pH 7.5, 30°C | Escherichia coli | |
| 5.4.2.6 | 0.5 | - |
beta-D-glucose 6-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 5.4.2.6 | 21 | - |
beta-D-glucose 1-phosphate | pH 8.0, 30°C | Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.230 | 7.5 | - |
assay at | Escherichia coli |
| 2.4.1.352 | 7.5 | - |
assay at | Escherichia coli |
| 5.4.2.6 | 8 | - |
assay at | Escherichia coli |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.230 | 1.12 | - |
2-alpha-D-glucosyl-D-glucose | pH 7.5, 30°C | Escherichia coli | |
| 2.4.1.352 | 0.16 | - |
phosphate | pH 7.5, 30°C | Escherichia coli | |
| 2.4.1.352 | 31 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | pH 7.5, 30°C | Escherichia coli | |
| 5.4.2.6 | 0.42 | - |
beta-D-glucose 6-phosphate | pH 8.0, 30°C | Escherichia coli | |
| 5.4.2.6 | 116 | - |
beta-D-glucose 1-phosphate | pH 8.0, 30°C | Escherichia coli | |
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