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Literature summary extracted from

  • Stroek, R.; Ge, Y.; Talbot, P.D.; Glok, M.K.; Bernas, K.E.; Thomson, C.M.; Gould, E.R.; Alphey, M.S.; Liu, H.; Florence, G.J.; Naismith, J.H.; da Silva, R.G.
    Kinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferase (2017), Biochemistry, 56, 793-803 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 expressed in Escherichia coli C43(DE3) cells Psychrobacter arcticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.17 hanging drop vapor diffusion method, using 11% (w/v) PEG 3350, 0.1 M bicine (pH 8.5), 0.15 M SrCl2, 0.15 M KBr, and 2% (v/v) 1,6-hexanediol Psychrobacter arcticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 AMP
-
 Psychrobacter arcticus
2.4.2.17 L-histidine
-
 Psychrobacter arcticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C Psychrobacter arcticus
2.4.2.17 0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C Psychrobacter arcticus
2.4.2.17 0.7
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Psychrobacter arcticus
2.4.2.17 0.7
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C Psychrobacter arcticus
2.4.2.17 0.8
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C Psychrobacter arcticus
2.4.2.17 1.2
-
 ATP at pH 8.5 and 30°C Psychrobacter arcticus
2.4.2.17 1.7
-
 ATP at pH 8.5 and 25°C Psychrobacter arcticus
2.4.2.17 1.8
-
 ATP at pH 8.5 and 20°C Psychrobacter arcticus
2.4.2.17 2
-
 ATP at pH 8.5 and 15°C Psychrobacter arcticus
2.4.2.17 2.1
-
 ATP at pH 8.5 and 10°C Psychrobacter arcticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+ dependent on, 10 mM used in assay conditions Psychrobacter arcticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Psychrobacter arcticus
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Psychrobacter arcticus DSM 17307
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Psychrobacter arcticus
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Psychrobacter arcticus DSM 17307
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Psychrobacter arcticus Q4FQF7
-
-
2.4.2.17 Psychrobacter arcticus DSM 17307 Q4FQF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 HisTrap column chromatography Psychrobacter arcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Psychrobacter arcticus ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Psychrobacter arcticus DSM 17307 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Psychrobacter arcticus 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Psychrobacter arcticus DSM 17307 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.17 heterooctamer 4 * 40000 + 4 * 23000, subunits HisZ and HisGS, SDS-PAGE Psychrobacter arcticus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 adenosine 5'-triphosphate phosphoribosyltransferase
-
 Psychrobacter arcticus
2.4.2.17 ATPPRT
-
 Psychrobacter arcticus
2.4.2.17 HisG
-
 Psychrobacter arcticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.17 38.9
-
 melting temperature for subunit HisZ Psychrobacter arcticus
2.4.2.17 42.9
-
 melting temperature for subunit HisGS Psychrobacter arcticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.17 0.45
-
 ATP at pH 8.5 and 10°C Psychrobacter arcticus
2.4.2.17 0.45
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C Psychrobacter arcticus
2.4.2.17 0.64
-
 ATP at pH 8.5 and 15°C Psychrobacter arcticus
2.4.2.17 0.64
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C Psychrobacter arcticus
2.4.2.17 0.94
-
 ATP at pH 8.5 and 30°C Psychrobacter arcticus
2.4.2.17 0.94
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C Psychrobacter arcticus
2.4.2.17 0.94
-
 ATP at pH 8.5 and 20°C Psychrobacter arcticus
2.4.2.17 0.94
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C Psychrobacter arcticus
2.4.2.17 1.16
-
 ATP at pH 8.5 and 25°C Psychrobacter arcticus
2.4.2.17 1.16
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Psychrobacter arcticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.17 0.214
-
 ATP at pH 8.5 and 10°C Psychrobacter arcticus
2.4.2.17 0.32
-
 ATP at pH 8.5 and 15°C Psychrobacter arcticus
2.4.2.17 0.422
-
 ATP at pH 8.5 and 20°C Psychrobacter arcticus
2.4.2.17 0.682
-
 ATP at pH 8.5 and 25°C Psychrobacter arcticus
2.4.2.17 0.75
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C Psychrobacter arcticus
2.4.2.17 0.783
-
 ATP at pH 8.5 and 30°C Psychrobacter arcticus
2.4.2.17 0.915
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C Psychrobacter arcticus
2.4.2.17 1.175
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C Psychrobacter arcticus
2.4.2.17 1.266
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C Psychrobacter arcticus
2.4.2.17 1.657
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Psychrobacter arcticus