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Literature summary extracted from

  • van Haren, M.J.; Sastre Torano, J.; Sartini, D.; Emanuelli, M.; Parsons, R.B.; Martin, N.I.
    A rapid and efficient assay for the characterization of substrates and inhibitors of nicotinamide N-methyltransferase (2016), Biochemistry, 55, 5307-5315 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.1 analysis development of a NNMT activity assay using ultra-high-performance hydrophilic interaction chromatography. It allows for rapid separation of the reaction products, coupled with quadrupole time-of-flight mass spectrometric detection for enhanced sensitivity and enabling high-throughput sample analysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.1 S-adenosyl-L-homocysteine
-
 Homo sapiens
2.1.1.1 sinefungin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.1 0.0085
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.087
-
 Isoquinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.111
-
 Thionicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.199
-
 nicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.335
-
 4-methylnicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.364
-
 quinoline 3-carboxamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.548
-
 2-methoxypyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.609
-
 Quinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.613
-
 tetrahydroisoquinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.727
-
 3-acetylpyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 1.326
-
 nicotinimidamide pH 8.6, 37°C Homo sapiens
2.1.1.1 2.029
-
 2-methylpyridine pH 8.6, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.1 Homo sapiens P40261
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.1 S-adenosyl-L-methionine + 2-methoxypyridine
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methyl-2-methoxypyridinium
-
 ?
2.1.1.1 S-adenosyl-L-methionine + 2-methylpyridine
-
 Homo sapiens S-adenosyl-L-homocysteine + 1,2-dimethylpyridinium
-
 ?
2.1.1.1 S-adenosyl-L-methionine + 3-acetylpyridine
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methyl-3-acetylpyridinium
-
 ?
2.1.1.1 S-adenosyl-L-methionine + 4-methylnicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1,4-dimethylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + isoquinoline
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methylisoquinoline
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinimidamide
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methylnicotinimidamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + quinoline
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methylquinoline
-
 ?
2.1.1.1 S-adenosyl-L-methionine + quinoline 3-carboxamide
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methylquinoline 3-carboxamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + tetrahydroisoquinoline
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methyltetrahydroisoquinoline
-
 ?
2.1.1.1 S-adenosyl-L-methionine + thionicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylthionicotinamide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.1 0.002
-
 2-methoxypyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.007
-
 quinoline 3-carboxamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.01
-
 2-methylpyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.014
-
 3-acetylpyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.014
-
 nicotinimidamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.017
-
 Isoquinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.033
-
 Thionicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.045
-
 tetrahydroisoquinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.046
-
 Quinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.091
-
 4-methylnicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.102
-
 nicotinamide pH 8.6, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.1 0.0039
-
 pH 8.6, 37°C Homo sapiens sinefungin
2.1.1.1 0.026
-
 pH 8.6, 37°C Homo sapiens S-adenosyl-L-homocysteine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.1
-
 273 4-methylnicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.005
-
 2-methylpyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.005
-
 2-methoxypyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.01
-
 nicotinimidamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.02
-
 3-acetylpyridine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.02
-
 quinoline 3-carboxamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.074
-
 tetrahydroisoquinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.076
-
 Quinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.197
-
 Isoquinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.3
-
 Thionicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.51
-
 nicotinamide pH 8.6, 37°C Homo sapiens