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Literature summary extracted from

  • Pierce, B.S.; Subedi, B.P.; Sardar, S.; Crowell, J.K.
    The ''Gln-Type'' thiol dioxygenase from Azotobacter vinelandii is a 3-mercaptopropionic acid dioxygenase (2015), Biochemistry, 54, 7477-7490 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.20 11.4
-
 L-cysteine pH 8.0, 25°C Azotobacter vinelandii
1.13.11.20 26
-
 cysteamine pH 8.0, 25°C Azotobacter vinelandii
1.13.11.91 0.013
-
 3-Mercaptopropanoate pH 8.0, 25°C Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.20 Iron iron content is 0.8 mol of iron per mol of MDO. Addition of NO to 3-mercaptopropanoate-bound enzyme quantitatively yields an iron-nitrosyl species with EPR features consistent with a mononuclear (FeNO)7 site Azotobacter vinelandii
1.13.11.91 Iron iron content is 0.8 mol of iron per mol of MDO. Addition of NO to 3-mercaptopropanoate-bound enzyme quantitatively yields an iron-nitrosyl species with EPR features consistent with a mononuclear (FeNO)7 site Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.20 Azotobacter vinelandii
-
-
-
1.13.11.91 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.20
-
 Azotobacter vinelandii
1.13.11.91
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.20 3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Azotobacter vinelandii 3-sulfinopropanoate + H+
-
 ?
1.13.11.20 cysteamine + O2
-
 Azotobacter vinelandii ?
-
 ?
1.13.11.20 L-cysteine + O2
-
 Azotobacter vinelandii 3-sulfinoalanine
-
 ?
1.13.11.20 additional information enzyme exhibits a specificity for 3-mercaptopropanoate nearly 2 orders of magnitude greater than those for cysteine Azotobacter vinelandii ?
-
-
1.13.11.91 3-mercaptopropanoate + O2
-
 Azotobacter vinelandii 3-sulfinopropanoate
-
 ?
1.13.11.91 L-cysteine + O2 reaction of EC 1.13.11.20 Azotobacter vinelandii 3-sulfinoalanine
-
 ?
1.13.11.91 additional information enzyme exhibits a specificity for 3-mercaptopropanoate nearly 2 orders of magnitude greater than those for cysteine Azotobacter vinelandii ?
-
-

Subunits

EC Number Subunits Comment Organism
1.13.11.20 ? x * 22696, calculated from sequence, x * 23000, SDS-PAGE Azotobacter vinelandii
1.13.11.91 ? x * 22696, calculated from sequence, x * 23000, SDS-PAGE Azotobacter vinelandii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.20 0.29
-
 cysteamine pH 8.0, 25°C Azotobacter vinelandii
1.13.11.20 1.2
-
 L-cysteine pH 8.0, 25°C Azotobacter vinelandii
1.13.11.91 0.94
-
 3-Mercaptopropanoate pH 8.0, 25°C Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.91 8
-
-
 Azotobacter vinelandii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.20 11
-
 cysteamine pH 8.0, 25°C Azotobacter vinelandii
1.13.11.20 110
-
 L-cysteine pH 8.0, 25°C Azotobacter vinelandii
1.13.11.91 72
-
 3-Mercaptopropanoate pH 8.0, 25°C Azotobacter vinelandii