Any feedback?
Literature summary extracted from
- Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl group (2011), Biochemistry, 50, 7705-7709 .
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.2.1.105 | H260A | mutation drastically reduces catalytic activity | Escherichia coli |
| 1.2.1.105 | H260E | mutation drastically reduces catalytic activity | Escherichia coli |
| 1.2.1.105 | H260E/H298N | almost 20fold decrease in kcat/Km vlaue | Escherichia coli |
| 1.2.1.105 | H298A | mutation drastically reduces catalytic activity | Escherichia coli |
| 1.2.1.105 | H298D | mutant accepts unnatural substrate 2-oxovalerate | Escherichia coli |
| 1.2.1.105 | H298L | mutant accepts unnatural substrate 2-oxovalerate | Escherichia coli |
| 1.2.1.105 | H298T | mutant accepts unnatural substrate 2-oxovalerate | Escherichia coli |
| 1.2.1.105 | H298V | mutant accepts unnatural substrate 2-oxovalerate | Escherichia coli |
| 1.2.1.105 | additional information | engineering of the E1 component to accept substrates lacking the 5-carboxylate group. Residue H260 is required for substrate recognition, but H298 can be replaced by hydrophobic residues of similar molecular volume | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.105 | 0.0025 | - |
2-oxoglutarate | mutant H298T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |  |
| 1.2.1.105 | 0.00261 | - |
2-oxoglutarate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.00273 | - |
2-oxoglutarate | mutant H260E/H298N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.00341 | - |
2-oxoglutarate | mutant H298L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.0063 | - |
2-oxovalerate | mutant H298L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.00702 | - |
2-oxovalerate | mutant H298D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.00896 | - |
2-oxovalerate | mutant H298V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.0153 | - |
2-oxovalerate | mutant H298T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.0163 | - |
2-oxovalerate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.165 | - |
2-oxoglutarate | mutant H298V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.383 | - |
2-oxoglutarate | mutant H260E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.105 | 2-oxoglutarate + CoA + NAD+ | Escherichia coli | - |
succinyl-CoA + CO2 + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.105 | Escherichia coli | P0AFG3 | i.e. 2-oxoglutarate dehydrogenase E1 component SucA, cf. EC 1.2.4.2 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.105 | 2-oxoglutarate + CoA + NAD+ | - |
Escherichia coli | succinyl-CoA + CO2 + NADH | - |
? | |
| 1.2.1.105 | 2-oxovalerate + CoA + NAD+ | unnatural substrate, very poor substrate of wild-type | Escherichia coli | ? + CO2 + NADH | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.1.105 | SucA | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.105 | 0.0064 | - |
2-oxoglutarate | mutant H298T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.03 | - |
2-oxovalerate | mutant H298T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.032 | - |
2-oxoglutarate | mutant H260E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.076 | - |
2-oxovalerate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.094 | - |
2-oxovalerate | mutant H298L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.099 | - |
2-oxoglutarate | mutant H298V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.134 | - |
2-oxoglutarate | mutant H260E/H298N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.415 | - |
2-oxoglutarate | mutant H298L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.556 | - |
2-oxovalerate | mutant H298V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 1.24 | - |
2-oxovalerate | mutant H298D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 2.15 | - |
2-oxoglutarate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.2.1.105 | physiological function | hybrid complexes consisting of recombinant components of OGDHc and pyruvate dehydrogenase enzymes suggest that a different component is the gatekeeper for specificity for the two multienzyme complexes in bacteria, the E1 component for pyruvate, but the E2 component for 2-oxoglutarate | Escherichia coli |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.105 | 0.002 | - |
2-oxovalerate | mutant H298T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.0047 | - |
2-oxovalerate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.0084 | - |
2-oxoglutarate | mutant H260E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.015 | - |
2-oxovalerate | mutant H298L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.062 | - |
2-oxovalerate | mutant H298V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.18 | - |
2-oxovalerate | mutant H298D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 0.6 | - |
2-oxoglutarate | mutant H298V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 1.5 | - |
2-oxoglutarate | mutant H298T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 49.1 | - |
2-oxoglutarate | mutant H260E/H298N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 122 | - |
2-oxoglutarate | mutant H298L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.2.1.105 | 824 | - |
2-oxoglutarate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
