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Literature summary extracted from

  • Li, M.; Wei, Y.; Yin, J.; Lin, L.; Zhou, Y.; Hua, G.; Cao, P.; Ang, E.L.; Zhao, H.; Yuchi, Z.; Zhang, Y.
    Biochemical and structural investigation of taurine 2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense (2019), Biochem. J., 476, 1605-1619 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.55 gene AH68_00255, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Bifidobacterium catenulatum subsp. kashiwanohense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.55 enzyme in complex with pyridoxal 5'-phosphate and glutamate, hanging-drop vapor diffusion method, mixing of 15 mg/ml protein solution with reservoir solution containing 0.1 M MES, pH 5.0, 0.2 M ammonium chloride, 20% w/v PEG 6000, 0.5 mM PLP, and 100 mM glutamate, X-ray diffraction structure determination and analysis at 2.7 A resolution Bifidobacterium catenulatum subsp. kashiwanohense

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.55 D154A site-directed mutagenesis Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 R156A site-directed mutagenesis Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 W21A site-directed mutagenesis Bifidobacterium catenulatum subsp. kashiwanohense

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.55 taurine-PLP aldimine induced fit docking modelling. Lys279, a key residue in the pocket, is the receptor box center Bifidobacterium catenulatum subsp. kashiwanohense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.55 1.1
-
 taurine pH 7.2, 37°C, recombinant enzyme Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 1.8
-
 2-oxoglutarate pH 7.2, 37°C, recombinant enzyme Bifidobacterium catenulatum subsp. kashiwanohense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.55 109700
-
 recombinant enzyme, gel filtration Bifidobacterium catenulatum subsp. kashiwanohense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.55 taurine + 2-oxoglutarate Bifidobacterium catenulatum subsp. kashiwanohense 2-oxoglutarate is the physiological acceptor 2-sulfoacetaldehyde + L-glutamate
-
 ?
2.6.1.55 taurine + 2-oxoglutarate Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 2-oxoglutarate is the physiological acceptor 2-sulfoacetaldehyde + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.55 Bifidobacterium catenulatum subsp. kashiwanohense A0A0A7I435 isolated from human infant feces
-
2.6.1.55 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 A0A0A7I435 isolated from human infant feces
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.55 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off by TEV protease, followed by another step of nickel affinity chromatography, dialysis, and anion exchange chromatography Bifidobacterium catenulatum subsp. kashiwanohense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.55 additional information in the enzyme complex crystal structure, two glutamate molecules are bound in sites near the predicted active site and they may occupy a path for substrate entry and product release Bifidobacterium catenulatum subsp. kashiwanohense ?
-
-
2.6.1.55 additional information in the enzyme complex crystal structure, two glutamate molecules are bound in sites near the predicted active site and they may occupy a path for substrate entry and product release Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 ?
-
-
2.6.1.55 taurine + 2-oxoglutarate 2-oxoglutarate is the physiological acceptor Bifidobacterium catenulatum subsp. kashiwanohense 2-sulfoacetaldehyde + L-glutamate
-
 ?
2.6.1.55 taurine + 2-oxoglutarate the enzyme BkToa from Bifidobacterium kashiwanohense shows high specificity for 2-oxoglutarate as the amine acceptor, pyruvate does not function as acceptor Bifidobacterium catenulatum subsp. kashiwanohense 2-sulfoacetaldehyde + L-glutamate
-
 ?
2.6.1.55 taurine + 2-oxoglutarate 2-oxoglutarate is the physiological acceptor Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 2-sulfoacetaldehyde + L-glutamate
-
 ?
2.6.1.55 taurine + 2-oxoglutarate the enzyme BkToa from Bifidobacterium kashiwanohense shows high specificity for 2-oxoglutarate as the amine acceptor, pyruvate does not function as acceptor Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 2-sulfoacetaldehyde + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.55 homodimer 2 * 49600, about, sequence calculation Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 More each monomer exhibits a typical type I PLP-enzyme fold and conserved PLP-coordinating residues interacting with the pyridoxal 5'-phosphate (PLP) molecule Bifidobacterium catenulatum subsp. kashiwanohense

Synonyms

EC Number Synonyms Comment Organism
2.6.1.55 BkToa
-
 Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 taurine:2-oxoglutarate aminotransferase
-
 Bifidobacterium catenulatum subsp. kashiwanohense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.55 22
-
 assay at room temperature Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 35
-
 coupled assay with glutamate dehydrogenase/alanine dehydrogenase Bifidobacterium catenulatum subsp. kashiwanohense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.55 3.7
-
 2-oxoglutarate pH 7.2, 37°C, recombinant enzyme Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 3.7
-
 taurine pH 7.2, 37°C, recombinant enzyme Bifidobacterium catenulatum subsp. kashiwanohense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.55 8
-
-
 Bifidobacterium catenulatum subsp. kashiwanohense

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.55 pyridoxal 5'-phosphate each monomer of the homodimeric enzyme exhibits a typical type I PLP-enzyme fold and conserved PLP-coordinating residues interacting with the pyridoxal 5'-phosphate (PLP) molecule, binding structure in the active site, modelling, overview Bifidobacterium catenulatum subsp. kashiwanohense

General Information

EC Number General Information Comment Organism
2.6.1.55 evolution sequence comparisons and phylogenetic analysis of taurine:oxoglutarate aminotransferases and taurine:pyruvate aminotransferases, overview Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 metabolism BkToa is part of a gene cluster putatively involved in taurine nitrogen assimilation. In this pathway, isothionate is formed by a sulfoacetaldehyde reductase TauF, a member of the metal-dependent alcohol dehydrogenase family Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 additional information molecular docking reveals a role for active site residues Trp21 and Arg156, conserved in Toa enzymes, in interacting with the sulfonate group of taurine Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 physiological function taurine is thought to serve as a source of carbon, nitrogen and sulfur for certain gut bacteria, particularly for strict anaerobes residing in the nutrient-poor environment of the distal gut. Taurine aminotransferases catalyze the first step in taurine catabolism in many taurine-degrading bacteria and play an important role in bacterial taurine metabolism in the mammalian gut Bifidobacterium catenulatum subsp. kashiwanohense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.55 2.06
-
 2-oxoglutarate pH 7.2, 37°C, recombinant enzyme Bifidobacterium catenulatum subsp. kashiwanohense
2.6.1.55 3.36
-
 taurine pH 7.2, 37°C, recombinant enzyme Bifidobacterium catenulatum subsp. kashiwanohense