EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.2.7 | synthesis | purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8) | Thermus thermophilus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.7 | recombinant expression of APRT in Escherichia coli strain BL21(DE3)/pER | Thermus thermophilus |
2.4.2.8 | recombinant expression of HPRT in Escherichia coli strain C3030/pER | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 0.013 | - |
hypoxanthine | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus | |
2.4.2.8 | 0.028 | - |
guanine | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus | |
2.4.2.8 | 0.22 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.7 | Mg2+ | the presence of magnesium ions promotes the proper spatial orientation of the substrate | Thermus thermophilus | |
2.4.2.8 | Mg2+ | the presence of magnesium ions promotes the proper spatial orientation of the substrate | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.7 | AMP + diphosphate | Thermus thermophilus | - |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Thermus thermophilus | - |
IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Thermus thermophilus DSM 7039 | - |
IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Thermus thermophilus ATCC BAA-163 | - |
IMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.7 | Thermus thermophilus | Q72I82 | - |
- |
2.4.2.8 | Thermus thermophilus | Q72GT2 | - |
- |
2.4.2.8 | Thermus thermophilus ATCC BAA-163 | Q72GT2 | - |
- |
2.4.2.8 | Thermus thermophilus DSM 7039 | Q72GT2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.7 | recombinant APRT from Escherichia coli strain BL21(DE3)/pER by hydrophobic interaction chromatography and ultrafiltration | Thermus thermophilus |
2.4.2.8 | recombinant HPRT from Escherichia coli strain C3030/pER by hydrophobic interaction chromatography and ultrafiltration | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.7 | 2,6-diaminopurine 5-phosphoribosyl nucleotide + diphosphate | low activity | Thermus thermophilus | 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | 2-aminobenzimidazole 5'-phosphoribosyl nucleotide + diphosphate | very low activity | Thermus thermophilus | 2-aminobenzimidazole + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | 2-chloro-AMP + diphosphate | best substrate | Thermus thermophilus | 2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | 2-fluoro-AMP + diphosphate | - |
Thermus thermophilus | 2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | 2-methoxyadenosine 5'-phosphate + diphosphate | - |
Thermus thermophilus | 2-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus | ? | - |
r | |
2.4.2.7 | AMP + diphosphate | - |
Thermus thermophilus | adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | additional information | purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No or poor activity with 8-azaguanine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide | Thermus thermophilus | ? | - |
- |
|
2.4.2.7 | N1-methyladenosine 5'-phosphate + diphosphate | high activity | Thermus thermophilus | N1-methyladenosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.7 | N6-benzyladenosine 5'-phosphate + diphosphate | very low activity | Thermus thermophilus | N6-benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.8 | 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus | 6-mercaptopurine riboside 5'-phosphate + diphosphate | - |
? | |
2.4.2.8 | 8-azaguanine + 5-phospho-alpha-D-ribose 1-diphosphate | high activity | Thermus thermophilus | 8-aza-IMP + diphosphate | - |
? | |
2.4.2.8 | adenine + 5-phospho-alpha-D-ribose 1-diphosphate | low activity | Thermus thermophilus | AMP + diphosphate | - |
? | |
2.4.2.8 | adenine + 5-phospho-alpha-D-ribose 1-diphosphate | low activity | Thermus thermophilus DSM 7039 | AMP + diphosphate | - |
? | |
2.4.2.8 | adenine + 5-phospho-alpha-D-ribose 1-diphosphate | low activity | Thermus thermophilus ATCC BAA-163 | AMP + diphosphate | - |
? | |
2.4.2.8 | allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus | ? | - |
? | |
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus | GMP + diphosphate | - |
? | |
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus DSM 7039 | GMP + diphosphate | - |
? | |
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus ATCC BAA-163 | GMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus | IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | best substrate | Thermus thermophilus | IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus DSM 7039 | IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | best substrate | Thermus thermophilus DSM 7039 | IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermus thermophilus ATCC BAA-163 | IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | best substrate | Thermus thermophilus ATCC BAA-163 | IMP + diphosphate | - |
? | |
2.4.2.8 | additional information | purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide | Thermus thermophilus | ? | - |
- |
|
2.4.2.8 | additional information | purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide | Thermus thermophilus DSM 7039 | ? | - |
- |
|
2.4.2.8 | additional information | purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide | Thermus thermophilus ATCC BAA-163 | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.7 | TthAPRT | - |
Thermus thermophilus |
2.4.2.7 | TT_C1250 | - |
Thermus thermophilus |
2.4.2.8 | TthHPRT | - |
Thermus thermophilus |
2.4.2.8 | TT_C1766 | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.7 | 75 | - |
- |
Thermus thermophilus |
2.4.2.8 | 60 | - |
- |
Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 2 | - |
guanine | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus | |
2.4.2.8 | 6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus | |
2.4.2.8 | 9 | - |
hypoxanthine | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.7 | 8 | - |
assay at | Thermus thermophilus |
2.4.2.8 | 8 | - |
assay at | Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 27.27 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus | |
2.4.2.8 | 71.4 | - |
guanine | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus | |
2.4.2.8 | 692.3 | - |
hypoxanthine | recombinant enzyme, pH 8.0, 70°C | Thermus thermophilus |