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Literature summary extracted from

  • Fateev, I.V.; Sinitsina, E.V.; Bikanasova, A.U.; Kostromina, M.A.; Tuzova, E.S.; Esipova, L.V.; Muravyova, T.I.; Kayushin, A.L.; Konstantinova, I.D.; Esipov, R.S.
    Thermophilic phosphoribosyltransferases Thermus thermophilus HB27 in nucleotide synthesis (2018), Beilstein J. Org. Chem., 14, 3098-3105 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.2.7 synthesis purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8) Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 recombinant expression of APRT in Escherichia coli strain BL21(DE3)/pER Thermus thermophilus
2.4.2.8 recombinant expression of HPRT in Escherichia coli strain C3030/pER Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.013
-
hypoxanthine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
2.4.2.8 0.028
-
guanine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
2.4.2.8 0.22
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 70°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+ the presence of magnesium ions promotes the proper spatial orientation of the substrate Thermus thermophilus
2.4.2.8 Mg2+ the presence of magnesium ions promotes the proper spatial orientation of the substrate Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 AMP + diphosphate Thermus thermophilus
-
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus DSM 7039
-
IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus ATCC BAA-163
-
IMP + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Thermus thermophilus Q72I82
-
-
2.4.2.8 Thermus thermophilus Q72GT2
-
-
2.4.2.8 Thermus thermophilus ATCC BAA-163 Q72GT2
-
-
2.4.2.8 Thermus thermophilus DSM 7039 Q72GT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 recombinant APRT from Escherichia coli strain BL21(DE3)/pER by hydrophobic interaction chromatography and ultrafiltration Thermus thermophilus
2.4.2.8 recombinant HPRT from Escherichia coli strain C3030/pER by hydrophobic interaction chromatography and ultrafiltration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 2,6-diaminopurine 5-phosphoribosyl nucleotide + diphosphate low activity Thermus thermophilus 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 2-aminobenzimidazole 5'-phosphoribosyl nucleotide + diphosphate very low activity Thermus thermophilus 2-aminobenzimidazole + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 2-chloro-AMP + diphosphate best substrate Thermus thermophilus 2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 2-fluoro-AMP + diphosphate
-
Thermus thermophilus 2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 2-methoxyadenosine 5'-phosphate + diphosphate
-
Thermus thermophilus 2-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ?
-
r
2.4.2.7 AMP + diphosphate
-
Thermus thermophilus adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No or poor activity with 8-azaguanine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus ?
-
-
2.4.2.7 N1-methyladenosine 5'-phosphate + diphosphate high activity Thermus thermophilus N1-methyladenosine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.7 N6-benzyladenosine 5'-phosphate + diphosphate very low activity Thermus thermophilus N6-benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.8 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus 6-mercaptopurine riboside 5'-phosphate + diphosphate
-
?
2.4.2.8 8-azaguanine + 5-phospho-alpha-D-ribose 1-diphosphate high activity Thermus thermophilus 8-aza-IMP + diphosphate
-
?
2.4.2.8 adenine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Thermus thermophilus AMP + diphosphate
-
?
2.4.2.8 adenine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Thermus thermophilus DSM 7039 AMP + diphosphate
-
?
2.4.2.8 adenine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Thermus thermophilus ATCC BAA-163 AMP + diphosphate
-
?
2.4.2.8 allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ?
-
?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus GMP + diphosphate
-
?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus DSM 7039 GMP + diphosphate
-
?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ATCC BAA-163 GMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate best substrate Thermus thermophilus IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus DSM 7039 IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate best substrate Thermus thermophilus DSM 7039 IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus ATCC BAA-163 IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate best substrate Thermus thermophilus ATCC BAA-163 IMP + diphosphate
-
?
2.4.2.8 additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus ?
-
-
2.4.2.8 additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus DSM 7039 ?
-
-
2.4.2.8 additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus ATCC BAA-163 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 TthAPRT
-
Thermus thermophilus
2.4.2.7 TT_C1250
-
Thermus thermophilus
2.4.2.8 TthHPRT
-
Thermus thermophilus
2.4.2.8 TT_C1766
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 75
-
-
Thermus thermophilus
2.4.2.8 60
-
-
Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 2
-
guanine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
2.4.2.8 6
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
2.4.2.8 9
-
hypoxanthine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 8
-
assay at Thermus thermophilus
2.4.2.8 8
-
assay at Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.8 27.27
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
2.4.2.8 71.4
-
guanine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
2.4.2.8 692.3
-
hypoxanthine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus