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Literature summary extracted from

  • Pandey, R.; Jung, H.; Parajuli, P.; Nguyen, T.; Bashyal, P.; Sohng, J.
    A synthetic approach for biosynthesis of miquelianin and scutellarin A in Escherichia coli (2019), Appl. Sci., 9, 215 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B71 functional recombinant overexpression of VvGT5 in Escherichia coli strain BL21 (DE3), coexpression with Veronica persica flavonoid 7-O-specific glucuronosyltransferase UGT88D8, subcloning in Escherichia coli strain XL-1 Blue Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B71 additional information coexpression of Vitis vinifera glucuronosyltransferase VvGT5 catalyzing the 3-O-specific glucuronic acid conjugation, and Veronica persica flavonoid 7-O-specific glucuronosyltransferase UGT88D8 to produce miquelianin and scutellarin A in Escherichia coli. Both plant UGTs are codon optimized, synthesized, and functionally expressed in Escherichia coli. Both genes are incorporated in single vector multi-monocistronic systems, overexpressing UDP-glucoronic pathway genes to develop two independent systems for regiospecific glycosylation Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B71 UDP-glucuronate + a flavonol Vitis vinifera
-
 UDP + a flavonol 3-O-beta-D-glucuronoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B71 Vitis vinifera
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B71 quercetin + UDP-glucuronate
-
 Vitis vinifera quercetin 3-O-glucuronate + UDP
-
 ?
2.4.1.B71 UDP-glucuronate + a flavonol
-
 Vitis vinifera UDP + a flavonol 3-O-beta-D-glucuronoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B71 ? x * 50920, sequence calculation Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B71 flavonoid 3-O-glucuronosyltransferase
-
 Vitis vinifera
2.4.1.B71 VvGT5
-
 Vitis vinifera

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.B71 Vitis vinifera sequence calculation
-
 5.21

General Information

EC Number General Information Comment Organism
2.4.1.B71 metabolism the enzyme is part of the UDP-glucuronic acid biosynthesis pathway Vitis vinifera
2.4.1.B71 physiological function Vitis vinifera glucuronosyltransferase VvGT5 catalyzes the 3-O-specific glucuronic acid conjugation Vitis vinifera