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Literature summary extracted from

  • Koonthongkaew, J.; Toyokawa, Y.; Ohashi, M.; Large, C.R.L.; Dunham, M.J.; Takagi, H.
    Effect of the Ala234Asp replacement in mitochondrial branched-chain amino acid aminotransferase on the production of BCAAs and fusel alcohols in yeast (2020), Appl. Microbiol. Biotechnol., 104, 7915-7925 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 recombinant expression of C-terminally GFP-tagged wild-type Bat1 and A234D mutant Bat1 in the Bat1-deletion strain Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.42 A234D site-directed mutagenesis of strain BY4741, both deletion mutant bat1DELTA and point mutant bat1A234D cells exhibit the same phenotypes relative to the wild-type Bat1 strain, namely, a repressive growth rate in the logarithmic phase, decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively, and an increase in fusel alcohol content in culture medium, and a decrease in the carbon dioxide productivity. These results indicate that amino acid change from Ala to Asp at position 234 leads to a functional impairment of Bat1, although homology modeling suggests that Asp234 in the variant Bat1 does not inhibit enzymatic activity directly Saccharomyces cerevisiae
2.6.1.42 additional information generation of a BY4741 bat1 deletion strain, phenotype, overview Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Saccharomyces cerevisiae
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate Saccharomyces cerevisiae ATCC 204508
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Saccharomyces cerevisiae
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Saccharomyces cerevisiae ATCC 204508
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Saccharomyces cerevisiae
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate Saccharomyces cerevisiae ATCC 204508
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Saccharomyces cerevisiae P38891
-
-
2.6.1.42 Saccharomyces cerevisiae ATCC 204508 P38891
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Saccharomyces cerevisiae 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Saccharomyces cerevisiae ATCC 204508 3-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Saccharomyces cerevisiae 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Saccharomyces cerevisiae ATCC 204508 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Saccharomyces cerevisiae 3-methyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Saccharomyces cerevisiae ATCC 204508 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 Bat1
-
 Saccharomyces cerevisiae
2.6.1.42 BCAA
-
 Saccharomyces cerevisiae
2.6.1.42 branched-chain amino acid aminotransferase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.6.1.42 malfunction the phenotypes of mutant A234D and bat1 deletion mutant are similar with a repressive growth rate in the logarithmic phase, decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively, an increase in fusel alcohol content in culture medium, and a decrease in the carbon dioxide productivity, overview. Effect of hyperosmotic stress on yeast cells expressing Bat1 Saccharomyces cerevisiae
2.6.1.42 metabolism inside the yeast mitochondria, both valine (Val) and leucine (Leu) are primarily biosynthesized from two pyruvates while isoleucine (Ile) is produced from an initial alpha-ketobutyrate molecule. Pyruvate and alpha-ketobutyrate are then converted into alpha-ketoisovalerate (KIV) and alpha-keto-beta-methylvalerate (KMV), respectively, through the same pathway. In contrast, alpha-ketoisocaproate (KIC) is synthesized from KIV. KIV, KMV, and KIC are finally transaminated to Val, Ile, and Leu, respectively, by the mitochondrial and cytoplasmic BCAA aminotransferases (BCATs) Bat1 and Bat2, respectively Saccharomyces cerevisiae
2.6.1.42 physiological function the mitochondrial branched-chain amino acid (BCAA) aminotransferase Bat1 plays an important role in the synthesis of branched-chain amino acids, i.e. valine, leucine, and isoleucine Saccharomyces cerevisiae