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Literature summary extracted from

  • Wang, T.T.; Zhu, C.Y.; Zheng, S.; Meng, C.C.; Wang, T.T.; Meng, D.H.; Li, Y.J.; Zhu, H.M.; Wang, F.S.; Sheng, J.Z.
    Identification and characterization of a chondroitin synthase from Avibacterium paragallinarum (2018), Appl. Microbiol. Biotechnol., 102, 4785-4797 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.175 gene acbD, encoded within the presumed capsule biosynthesis gene cluster, functional recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein Avibacterium paragallinarum
2.4.1.226 gene acbD, encoded within the presumed capsule biosynthesis gene cluster, functional recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein Avibacterium paragallinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.175 Cu2+
-
Avibacterium paragallinarum
2.4.1.175 Fe3+
-
Avibacterium paragallinarum
2.4.1.175 Ni2+
-
Avibacterium paragallinarum
2.4.1.175 Zn2+
-
Avibacterium paragallinarum
2.4.1.226 Cu2+
-
Avibacterium paragallinarum
2.4.1.226 Fe3+
-
Avibacterium paragallinarum
2.4.1.226 Ni2+
-
Avibacterium paragallinarum
2.4.1.226 Zn2+
-
Avibacterium paragallinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.175 additional information
-
additional information Michaelis-Menten kinetics Avibacterium paragallinarum
2.4.1.175 9.275
-
UDP-GalNAc pH 7.0, 25°C Avibacterium paragallinarum
2.4.1.226 additional information
-
additional information Michaelis-Menten kinetics Avibacterium paragallinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.175 Ca2+ activates slightly Avibacterium paragallinarum
2.4.1.175 Mg2+ activates moderately Avibacterium paragallinarum
2.4.1.175 Mn2+ best activating metal ion Avibacterium paragallinarum
2.4.1.226 Ca2+ activates slightly Avibacterium paragallinarum
2.4.1.226 Mg2+ activates moderately Avibacterium paragallinarum
2.4.1.226 Mn2+ best activating metal ion Avibacterium paragallinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.226 NDP-glucose + 3-phospho-D-glycerate Avibacterium paragallinarum
-
NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.226 NDP-glucose + 3-phospho-D-glycerate Avibacterium paragallinarum ATCC 29545
-
NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.226 NDP-glucose + 3-phospho-D-glycerate Avibacterium paragallinarum NCTC 11296
-
NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.175 Avibacterium paragallinarum D0QYM5 genotype A
-
2.4.1.175 Avibacterium paragallinarum ATCC 29545 D0QYM5 genotype A
-
2.4.1.175 Avibacterium paragallinarum NCTC 11296 D0QYM5 genotype A
-
2.4.1.226 Avibacterium paragallinarum D0QYM5 genotype A
-
2.4.1.226 Avibacterium paragallinarum ATCC 29545 D0QYM5 genotype A
-
2.4.1.226 Avibacterium paragallinarum NCTC 11296 D0QYM5 genotype A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.175 recombinant soluble N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Avibacterium paragallinarum
2.4.1.226 recombinant soluble N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Avibacterium paragallinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.175 additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview. GlcA-pNP can serve as the acceptor for ApCS Avibacterium paragallinarum ?
-
-
2.4.1.175 additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview. GlcA-pNP can serve as the acceptor for ApCS Avibacterium paragallinarum ATCC 29545 ?
-
-
2.4.1.175 additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview. GlcA-pNP can serve as the acceptor for ApCS Avibacterium paragallinarum NCTC 11296 ?
-
-
2.4.1.175 UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
Avibacterium paragallinarum UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
?
2.4.1.175 UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
Avibacterium paragallinarum ATCC 29545 UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
?
2.4.1.175 UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
Avibacterium paragallinarum NCTC 11296 UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
?
2.4.1.226 additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview Avibacterium paragallinarum ?
-
-
2.4.1.226 additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview Avibacterium paragallinarum ATCC 29545 ?
-
-
2.4.1.226 additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview Avibacterium paragallinarum NCTC 11296 ?
-
-
2.4.1.226 NDP-glucose + 3-phospho-D-glycerate
-
Avibacterium paragallinarum NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.226 NDP-glucose + 3-phospho-D-glycerate
-
Avibacterium paragallinarum ATCC 29545 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.226 NDP-glucose + 3-phospho-D-glycerate
-
Avibacterium paragallinarum NCTC 11296 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.226 UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
Avibacterium paragallinarum UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
?
2.4.1.226 UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
Avibacterium paragallinarum ATCC 29545 UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
?
2.4.1.226 UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
Avibacterium paragallinarum NCTC 11296 UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.175 ? x * 111000, about, sequence calculation, x * 100000-130000, recombinant His-tagged enzyme, SDS-PAGE Avibacterium paragallinarum
2.4.1.226 ? x * 111000, about, sequence calculation, x * 100000-130000, recombinant His-tagged enzyme, SDS-PAGE Avibacterium paragallinarum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.175 AcbD
-
Avibacterium paragallinarum
2.4.1.175 ApCS
-
Avibacterium paragallinarum
2.4.1.175 chondroitin synthase
-
Avibacterium paragallinarum
2.4.1.175 GalNAc-T
-
Avibacterium paragallinarum
2.4.1.175 More see also EC 2.4.1.226 Avibacterium paragallinarum
2.4.1.226 AcbD
-
Avibacterium paragallinarum
2.4.1.226 ApCS
-
Avibacterium paragallinarum
2.4.1.226 chondroitin synthase
-
Avibacterium paragallinarum
2.4.1.226 More see also EC 2.4.1.175 Avibacterium paragallinarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.175 25 37 assay at Avibacterium paragallinarum
2.4.1.226 25 37 assay at Avibacterium paragallinarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.175 additional information
-
ApCS is a cold-active enzyme Avibacterium paragallinarum
2.4.1.226 additional information
-
ApCS is a cold-active enzyme Avibacterium paragallinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.175 4 20 75% activity remaining after 10 h, 60% activity remaining after 70 h Avibacterium paragallinarum
2.4.1.175 30
-
60% activity remaining after 10 h, 40% activity remaining after 70 h Avibacterium paragallinarum
2.4.1.175 40 55 inactivation within 5 h Avibacterium paragallinarum
2.4.1.226 4 20 75% activity remaining after 10 h, 60% activity remaining after 70 h Avibacterium paragallinarum
2.4.1.226 30
-
60% activity remaining after 10 h, 40% activity remaining after 70 h Avibacterium paragallinarum
2.4.1.226 40 55 inactivation within 5 h Avibacterium paragallinarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.175 355
-
UDP-GalNAc pH 7.0, 25°C Avibacterium paragallinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.175 7
-
assay at Avibacterium paragallinarum
2.4.1.226 7
-
assay at Avibacterium paragallinarum

General Information

EC Number General Information Comment Organism
2.4.1.175 physiological function the chondroitin synthase (ApCS) is a bifunctional enzyme catalyzing the elongation of the chondroitin chain by alternatively transferring the glucuronic acid (GlcA) and N-acetyl-D-galactosamine (GalNAc) residues from their nucleotide forms to the non-reducing ends of the saccharide chains Avibacterium paragallinarum
2.4.1.226 physiological function the chondroitin synthase (ApCS) is a bifunctional enzyme catalyzing the elongation of the chondroitin chain by alternatively transferring the glucuronic acid (GlcA) and N-acetyl-D-galactosamine (GalNAc) residues from their nucleotide forms to the non-reducing ends of the saccharide chains Avibacterium paragallinarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.175 38.28
-
UDP-GalNAc pH 7.0, 25°C Avibacterium paragallinarum