EC Number | General Stability | Organism |
---|---|---|
1.17.99.10 | immobilization of the enzyme either on powder or a monolithic silica support functionalized with an organic linker providing NH2 groups for enzyme covalent binding extends the catalytic lifetime of the enzyme under working conditions at least 3.5times | Sterolibacterium denitrificans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.99.10 | Mo2+ | molybdenum enzyme | Sterolibacterium denitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.99.10 | cholecalciferol + acceptor + H2O | Sterolibacterium denitrificans | the product 25-hydroxycholecalciferol (calcifediol) is the activated form of vitamin D3 used in the treatment of rickets and other calcium disorders | 25-hydroxycholecalciferol + reduced acceptor | - |
? | |
1.17.99.10 | cholesterol + acceptor + H2O | Sterolibacterium denitrificans | the product 25-hydroxycholesterol is responsible for a complex regulatory function in the immunological system | 25-hydroxycholesterol + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.99.10 | Sterolibacterium denitrificans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.99.10 | - |
Sterolibacterium denitrificans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.99.10 | additional information | - |
immobilization of the enzyme either on powder or a monolithic silica support functionalized with an organic linker providing NH2 groups for enzyme covalent binding reduces the initial activity of the enzyme more than twofold | Sterolibacterium denitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.99.10 | cholecalciferol + acceptor + H2O | the product 25-hydroxycholecalciferol (calcifediol) is the activated form of vitamin D3 used in the treatment of rickets and other calcium disorders | Sterolibacterium denitrificans | 25-hydroxycholecalciferol + reduced acceptor | - |
? | |
1.17.99.10 | cholecalciferol + acceptor + H2O | 90% concversion after 46 h | Sterolibacterium denitrificans | 25-hydroxycholecalciferol + reduced acceptor | - |
? | |
1.17.99.10 | cholest-4-en-3-one + acceptor + H2O | 90% concversion after 4 h | Sterolibacterium denitrificans | 25-hydroxycholest-4-en-3-one + reduced acceptor | - |
? | |
1.17.99.10 | cholesta-1,4-dien-3-one + acceptor + H2O | 90% concversion after 4 h | Sterolibacterium denitrificans | 25-hydroxycholesta-1,4-dien-3-one + reduced acceptor | - |
? | |
1.17.99.10 | cholesterol + acceptor + H2O | - |
Sterolibacterium denitrificans | 25-hydroxycholesterol + reduced acceptor | - |
? | |
1.17.99.10 | cholesterol + acceptor + H2O | the product 25-hydroxycholesterol is responsible for a complex regulatory function in the immunological system | Sterolibacterium denitrificans | 25-hydroxycholesterol + reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.99.10 | S25DH | - |
Sterolibacterium denitrificans |
1.17.99.10 | steroid C25 dehydrogenase | - |
Sterolibacterium denitrificans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.99.10 | 30 | - |
assay at | Sterolibacterium denitrificans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.99.10 | 7 | - |
assay at | Sterolibacterium denitrificans |