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Literature summary extracted from

  • Milanes, J.E.; Suryadi, J.; Abendroth, J.; Van Voorhis, W.C.; Barrett, K.F.; Dranow, D.M.; Phan, I.Q.; Patrick, S.L.; Rozema, S.D.; Khalifa, M.M.; Golden, J.E.; Morris, J.C.
    Enzymatic and structural characterization of the Naegleria fowleri glucokinase (2019), Antimicrob. Agents Chemother., 63, e02410-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 expression in Escherichia coli Naegleria fowleri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.2 the structure is determined and refined to 2.2 A resolution Naegleria fowleri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 N,N'-[furan-2,5-diylbis(2-chloro-4,1-phenylene)]diguanidine
-
 Homo sapiens
2.7.1.2 4-(4-tert-butylbenzamido)-3',4',6-trichloro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
2.7.1.2 4-(4-tert-butylbenzamido)-3',6-dichloro-4'-fluoro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
2.7.1.2 4-(4-tert-butylbenzamido)-3',6-dichloro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
2.7.1.2 4-(4-tert-butylbenzamido)-6-chloro-3'-nitro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
2.7.1.2 4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethoxy)[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
2.7.1.2 4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethyl)[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
2.7.1.2 ADP
-
 Naegleria fowleri
2.7.1.2 ebselen
-
 Naegleria fowleri
2.7.1.2 glucosamine
-
 Naegleria fowleri
2.7.1.2 N,N'-[furan-2,5-diylbis(2-chloro-4,1-phenylene)]diguanidine
-
 Naegleria fowleri
2.7.1.2 N,N'-[furan-2,5-diylbis(3-chloro-4,1-phenylene)]diguanidine
-
 Naegleria fowleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.0425
-
 D-glucose pH 7.4, 22°C Naegleria fowleri
2.7.1.2 0.1416
-
 ATP pH 7.4, 22°C Naegleria fowleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 CaCl2 MnCl2 yields 9% of the activity observed when MgCl2 is included in the assay Naegleria fowleri
2.7.1.2 MgCl2 the enzyme functions best when MgCl2 is included in the assay Naegleria fowleri
2.7.1.2 MnCl2 MnCl2 yields 40% of the activity observed when MgCl2 is included in the assay Naegleria fowleri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Homo sapiens
-
 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose Naegleria fowleri
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Homo sapiens P35557
-
-
2.7.1.2 Naegleria fowleri A0A384E136
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2
-
 Naegleria fowleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucosamine
-
 Naegleria fowleri ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose
-
 Naegleria fowleri ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose limited hexose substrate range, with the greatest activity observed with glucose Naegleria fowleri ADP + D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 NfGlck
-
 Homo sapiens
2.7.1.2 NfGlck
-
 Naegleria fowleri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.2 36.5
-
 ATP pH 7.4, 22°C Naegleria fowleri
2.7.1.2 36.5
-
 D-glucose pH 7.4, 22°C Naegleria fowleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 8.5
-
-
 Naegleria fowleri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.2 7.5 9.5 pH 7.5: about 65% of maximal activity, pH 9.5: about 30% of maximal activity Naegleria fowleri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.2 0.116
-
 ADP pH 7.4, 22°C Naegleria fowleri
2.7.1.2 0.1864
-
 glucosamine pH 7.4, 22°C Naegleria fowleri

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.1 0.00026
-
 pH and temperature not specified in the publication Homo sapiens N,N'-[furan-2,5-diylbis(2-chloro-4,1-phenylene)]diguanidine
2.7.1.2 0.0007
-
 pH and temperature not specified in the publication Naegleria fowleri N,N'-[furan-2,5-diylbis(2-chloro-4,1-phenylene)]diguanidine
2.7.1.2 0.00088
-
 pH and temperature not specified in the publication Naegleria fowleri ebselen
2.7.1.2 0.002
-
 pH and temperature not specified in the publication Naegleria fowleri N,N'-[furan-2,5-diylbis(3-chloro-4,1-phenylene)]diguanidine
2.7.1.2 0.0029
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-3',4',6-trichloro[1,1'-biphenyl]-3-carboxylic acid
2.7.1.2 0.005
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethoxy)[1,1'-biphenyl]-3-carboxylic acid
2.7.1.2 0.0059
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-3',6-dichloro-4'-fluoro[1,1'-biphenyl]-3-carboxylic acid
2.7.1.2 0.006
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethyl)[1,1'-biphenyl]-3-carboxylic acid
2.7.1.2 0.0065
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-3',6-dichloro[1,1'-biphenyl]-3-carboxylic acid
2.7.1.2 0.0093
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-6-chloro-3'-nitro[1,1'-biphenyl]-3-carboxylic acid

General Information

EC Number General Information Comment Organism
2.7.1.2 metabolism the enzyme plays a role in metabolism of nutrients acquired from the environment through either predation or directly from the sediment Naegleria fowleri