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Literature summary extracted from

  • Sun, H.; Yeo, W.L.; Lim, Y.H.; Chew, X.; Smith, D.J.; Xue, B.; Chan, K.P.; Robinson, R.C.; Robins, E.G.; Zhao, H.; Ang, E.L.
    Directed evolution of a fluorinase for improved fluorination efficiency with a non-native substrate (2016), Angew. Chem. Int. Ed. Engl., 55, 14277-14280 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.63 A279Y about 3fold improved conversion of the non-native substrate 5'-deoxy-5'-chloroadenosine into 5'-fluoro-5'-deoxyadenosine. Mutant displays an improved kcat value in the conversion of 5'-deoxy-5'-chloroadenosine into S-adenosyl-L-methionine but a reduced kcat value in the conversion of S-adenosyl-L-methionine into 5'-fluoro-5'-deoxyadenosine Streptomyces sp. MA37
2.5.1.63 F213Y/A279L about 3fold improved conversion of the non-native substrate 5'-deoxy-5'-chloroadenosine into 5'-fluoro-5'-deoxyadenosine. Mutant displays an improved kcat value in the conversion of 5'-deoxy-5'-chloroadenosine into S-adenosyl-L-methionine but a reduced kcat value in the conversion of S-adenosyl-L-methionine into 5'-fluoro-5'-deoxyadenosine Streptomyces sp. MA37

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.0084
-
 5'-deoxy-5'-chloroadenosine wild-type, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0092
-
 S-adenosyl-L-methionine mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0095
-
 5'-deoxy-5'-chloroadenosine mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0222
-
 S-adenosyl-L-methionine mutant A279Y, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0346
-
 S-adenosyl-L-methionine wild-type, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0593
-
 5'-deoxy-5'-chloroadenosine mutant A279Y, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces sp. MA37 W0W999
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 5'-deoxy-5'-chloroadenosine + L-methionine
-
 Streptomyces sp. MA37 S-adenosyl-L-methionine + chloride
-
 r
2.5.1.63 5'-deoxy-5'-chloroadenosine + L-selenomethionine use of L-selenomethionine increases the yield by around 2fold Streptomyces sp. MA37 S-adenosyl-L-selenomethionine + chloride
-
 r
2.5.1.63 additional information by coupling the reactions, conversion of 5'-deoxy-5'-chloroadenosine to 5'-fluoro-5'-deoxyadenosine can be achieved Streptomyces sp. MA37 ?
-
-
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces sp. MA37 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 FlA1
-
 Streptomyces sp. MA37

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 42
-
-
 Streptomyces sp. MA37

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.63 0.0003
-
 S-adenosyl-L-methionine mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0018
-
 S-adenosyl-L-methionine mutant A279Y, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0022
-
 5'-deoxy-5'-chloroadenosine wild-type, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0037
-
 S-adenosyl-L-methionine wild-type, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0088
-
 5'-deoxy-5'-chloroadenosine mutant A279Y, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.0643
-
 5'-deoxy-5'-chloroadenosine mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.63 0.043
-
 S-adenosyl-L-methionine mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.08
-
 S-adenosyl-L-methionine mutant A279Y, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.108
-
 S-adenosyl-L-methionine wild-type, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.149
-
 5'-deoxy-5'-chloroadenosine mutant A279Y, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 0.268
-
 5'-deoxy-5'-chloroadenosine wild-type, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37
2.5.1.63 6.798
-
 5'-deoxy-5'-chloroadenosine mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication Streptomyces sp. MA37