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Literature summary extracted from

  • Lei, B.; Xie, L.; Zhang, S.; Lv, D.; Shu, F.; Deng, Y.
    UBE2W down-regulation promotes cell apoptosis and correlates with hypospermatogenesis (2020), Andrologia, 52, e13474 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.2.25 diagnostics UBE2W downregulation promotes cell apoptosis and correlates with hypospermatogenesis, which may be helpful for the diagnosis of male infertility Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.25 gene Ube2w, recombinant expression of a recombinant lentivirus containing murine UBE2W Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.25 additional information specific downregulation of enzyme UBE2W in spermatogenic cells by murine UBE2W-specific shRNA. UBE2W downregulation promotes cell apoptosis and correlates with hypospermatogenesis. UBE2W upregulation is performed by a recombinant lentivirus containing murine UBE2W Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.25 cytoplasm
-
 Mus musculus 5737
-
2.3.2.25 nucleoplasm
-
 Mus musculus 5654
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.25 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid Mus musculus
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein]
-
 ?
2.3.2.25 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid Mus musculus C57
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.25 Mus musculus Q8VDW4
-
-
2.3.2.25 Mus musculus C57 Q8VDW4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.25 GC-1 spg cell a spermatogonia cell line Mus musculus
-
2.3.2.25 GC-2 cell a spermatocyte cell line Mus musculus
-
2.3.2.25 spermatocyte
-
 Mus musculus
-
2.3.2.25 spermatogonium
-
 Mus musculus
-
2.3.2.25 testis the expression levels of UBE2W in mouse testes are gradually elevated from 2 to 10 weeks, but they are significantly deceased in the testes with hypospermatogenesis Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.25 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid
-
 Mus musculus [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein]
-
 ?
2.3.2.25 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid
-
 Mus musculus C57 [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein]
-
 ?
2.3.2.25 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [Rnf4]-N-terminal-amino acid
-
 Mus musculus [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[Rnf4]
-
 ?
2.3.2.25 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [Rnf4]-N-terminal-amino acid
-
 Mus musculus C57 [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[Rnf4]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.25 UBE2W
-
 Mus musculus
2.3.2.25 ubiquitin conjugating enzyme E2
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.2.25 malfunction the expression levels of UBE2W in mouse testes are significantly deceased in the testes with hypospermatogenesis. When UBE2W expression is successfully downregulated in spermatogenic cells, the rate of apoptosis is significantly increased and the P53/Bcl-2/caspase 6/caspase 9 signal pathways are activated. UBE2W downregulation promotes cell apoptosis and correlates with hypospermatogenesis Mus musculus
2.3.2.25 metabolism UBE2W expression correlates with testis development and hypospermatogenesis. UBE2W expression affects cell apoptosis by P53/Bcl-2/caspase 6/caspase 9 signal pathways, quantitative RT-PCR expression analysis, overview Mus musculus
2.3.2.25 physiological function ubiquitin conjugating enzyme (E2) is crucial for mediating N-terminal ubiquitination. Enzyme UBE2W is involved in male infertility, correlation between UBE2W expression and hypospermatogenesis, overview. UBE2W promotes ubiquitin chain formation in response to DNA damage by interacting with Rnf4 Mus musculus