EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.1 | 8-Bromoadenosine | about 30% inhibition | Homo sapiens | |
2.4.2.1 | 8-Bromoguanosine | about 30% inhibition | Homo sapiens | |
2.4.2.1 | 8-Bromoguanosine | about 25% inhibition | Mycobacterium tuberculosis | |
2.4.2.1 | 8-dimethylaminoinosine | about 20% inhibition | Homo sapiens | |
2.4.2.1 | 8-methoxyinosine | about 20% inhibition | Mycobacterium tuberculosis | |
2.4.2.1 | 8-methylaminoinosine | about 30% inhibition | Homo sapiens | |
2.4.2.1 | 8-methylaminoinosine | about 25% inhibition | Mycobacterium tuberculosis | |
2.4.2.1 | acyclovir | about 40% inhibition | Homo sapiens | |
2.4.2.1 | acyclovir | about 35% inhibition | Mycobacterium tuberculosis | |
2.4.2.1 | additional information | development, validation, and application of a 96-well enzymatic assay based on LC-ESI-MS/MS quantification for the screening of selective inhibitors against Mycobacterium tuberculosis purine nucleoside phosphorylase, overview. Evaluation of a set of 8-halo-, 8-amino-, 8-O-alkyl-substituted purine ribonucleosides synthesized on purpose as potential inhibitors against MtbPNP. The assayed 8-substituted ribonucleosides do not exert a significant inhibitory effect against the tested enzymes up to 1 mM. No or poor inhibition by formycin A (an inhibitor of hexameric PNP), 8-ethylaminoinosine, 8-methoxyinosine, and 8-ethoxyinosine | Homo sapiens | |
2.4.2.1 | additional information | development, validation, and application of a 96-well enzymatic assay based on LC-ESI-MS/MS quantification for the screening of selective inhibitors against Mycobacterium tuberculosis purine nucleoside phosphorylase, overview. Evaluation of a set of 8-halo-, 8-amino-, 8-O-alkyl-substituted purine ribonucleosides synthesized on purpose as potential inhibitors against MtbPNP. The assayed 8-substituted ribonucleosides do not exert a significant inhibitory effect against the tested enzymes up to 1 mM. No or poor inhibition by formycin A (an inhibitor of hexameric PNP), 8-bromoadenosine, 8-ethylaminoinosine, 8-dimethylaminoinosine, and 8-ethoxyinosine | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | additional information | - |
additional information | Michaelis-Menten kinetics | Mycobacterium tuberculosis | |
2.4.2.1 | additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
2.4.2.1 | 0.06 | - |
Inosine | pH 7.5, 37°C | Mycobacterium tuberculosis | |
2.4.2.1 | 0.15 | - |
phosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
2.4.2.1 | 0.15 | - |
Inosine | pH 7.5, 37°C | Homo sapiens | |
2.4.2.1 | 0.66 | - |
phosphate | pH 7.5, 37°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.1 | inosine + phosphate | Mycobacterium tuberculosis | complete conversion | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
2.4.2.1 | inosine + phosphate | Homo sapiens | complete conversion | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.1 | Homo sapiens | P00491 | - |
- |
2.4.2.1 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.1 | inosine + phosphate | complete conversion | Mycobacterium tuberculosis | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
2.4.2.1 | inosine + phosphate | complete conversion | Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.1 | homotrimer | - |
Mycobacterium tuberculosis |
2.4.2.1 | homotrimer | - |
Homo sapiens |
2.4.2.1 | More | the enzyme appears to have transition states | Mycobacterium tuberculosis |
2.4.2.1 | More | the enzyme appears to have transition states | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.1 | MtbPNP | - |
Mycobacterium tuberculosis |
2.4.2.1 | MtbPNP | - |
Homo sapiens |
2.4.2.1 | PNP | - |
Mycobacterium tuberculosis |
2.4.2.1 | PNP | - |
Homo sapiens |
2.4.2.1 | purine nucleoside phosphorylase | - |
Mycobacterium tuberculosis |
2.4.2.1 | purine nucleoside phosphorylase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 37 | - |
assay at | Mycobacterium tuberculosis |
2.4.2.1 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | 4.5 | - |
phosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
2.4.2.1 | 14 | - |
Inosine | pH 7.5, 37°C | Homo sapiens | |
2.4.2.1 | 15.7 | - |
Inosine | pH 7.5, 37°C | Mycobacterium tuberculosis | |
2.4.2.1 | 76 | - |
phosphate | pH 7.5, 37°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
2.4.2.1 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | additional information | - |
additional information | inhibition kinetics | Mycobacterium tuberculosis | |
2.4.2.1 | additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
2.4.2.1 | 0.028 | - |
acyclovir | pH 7.5, 37°C | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | 30 | - |
phosphate | pH 7.5, 37°C | Mycobacterium tuberculosis | |
2.4.2.1 | 93.3 | - |
Inosine | pH 7.5, 37°C | Homo sapiens | |
2.4.2.1 | 115.15 | - |
phosphate | pH 7.5, 37°C | Homo sapiens | |
2.4.2.1 | 261.7 | - |
Inosine | pH 7.5, 37°C | Mycobacterium tuberculosis |