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Literature summary extracted from

  • Balakrishnan, A.; Price, E.; Luu, C.; Shaul, J.; Wartchow, C.; Cantwell, J.; Vo, T.; DiDonato, M.; Spraggon, G.; Hekmat-Nejad, M.
    Biochemical characterization of respiratory syncytial virus RNA-dependent RNA polymerase complex (2020), ACS Infect. Dis., 6, 2800-2811 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.48 L-protein the intact L protein is essential for enzyme (RdRP) activity and optimal RNA binding. In truncated L protein constructs, enzyme activity is abrogated due to their deficiency in RNA template binding Respiratory syncytial virus type A

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 N812A active site variant of RSV L-protein in the RSV L/P complex. The RSV L(N812A)/P complex does not produce RNA products with TrC sequence RNA as template Respiratory syncytial virus type A

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 additional information not sensitive to actinomycin D Respiratory syncytial virus type A

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 0.00018
-
 CTP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
2.7.7.48 0.0011
-
 RNAn 30°C, pH 7.5, RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
2.7.7.48 0.0014
-
 UTP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
2.7.7.48 0.0027
-
 GTP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A
2.7.7.48 0.0085
-
 ATP 30°C, pH 7.5, cosubstrate: RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Respiratory syncytial virus type A the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Respiratory syncytial virus type A X5F520
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 ATP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
 ?
2.7.7.48 CTP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
 ?
2.7.7.48 GTP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome Respiratory syncytial virus type A diphosphate + RNAn+1
-
 ?
2.7.7.48 UTP + RNAn RNA template with the first 25 nucleotides from the TrC (Trailer complement) sequence Respiratory syncytial virus type A diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 More the core polymerase in respiratory syncytial virus (RSV) is composed of two proteins, a 250 kDa polymerase subunit (L), which contains the RNA-dependent RNA polymerase, the polyribonucleotidyl transferase (PRNTase, capping), and the methyltransferase enzymatic domains essential for viral transcription and replication, and the 27 kDa phosphoprotein (P) accessory protein Respiratory syncytial virus type A

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 L/P RdRP
-
 Respiratory syncytial virus type A
2.7.7.48 RNA-dependent RNA polymerase
-
 Respiratory syncytial virus type A

General Information

EC Number General Information Comment Organism
2.7.7.48 physiological function the enzyme (RdRP) is essential for both transcription and replication of the viral RNA genome Respiratory syncytial virus type A