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Literature summary extracted from

  • Nancolas, B.; Bull, I.D.; Stenner, R.; Dufour, V.; Curnow, P.
    Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a thioesterase in vitro (2017), Yeast, 34, 239-251 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.84 expression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.84 D195N mutant cannot be recombinantly expressed Saccharomyces cerevisiae
2.3.1.84 H191A about 50% decrease in acyl-CoA hydrolase activity Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.84 membrane
-
 Saccharomyces cerevisiae 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.84 140000
-
 gel filtration, protein-detergent complex comprising an Atf1 monomer of 65 kDa associated with the Thesit micelle Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.84 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.84
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.84 acetyl-CoA + butanol
-
 Saccharomyces cerevisiae CoA + butyl acetate
-
 ?
2.3.1.84 acetyl-CoA + ethanol
-
 Saccharomyces cerevisiae CoA + ethyl acetate
-
 ?
2.3.1.84 acetyl-CoA + heptanol
-
 Saccharomyces cerevisiae CoA + heptyl acetate
-
 ?
2.3.1.84 acetyl-CoA + hexanol
-
 Saccharomyces cerevisiae CoA + hexyl acetate
-
 ?
2.3.1.84 acetyl-CoA + isoamyl alcohol
-
 Saccharomyces cerevisiae CoA + isoamyl acetate
-
 ?
2.3.1.84 acetyl-CoA + isobutanol
-
 Saccharomyces cerevisiae CoA + isobutyl acetate
-
 ?
2.3.1.84 acetyl-CoA + octanol
-
 Saccharomyces cerevisiae CoA + octyl acetate
-
 ?
2.3.1.84 additional information Atf1 uses acetyl-CoA to synthesize acetate esters from all of the alcohols tested. Atf1 is specific for acetyl-CoA. Additionally, Atf1 can hydrolyse all of the acyl-CoA substrates tested, with a clear preference for C8 chain lengths Saccharomyces cerevisiae ?
-
-

Subunits

EC Number Subunits Comment Organism
2.3.1.84 ? x * 65000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.84 ATF1
-
 Saccharomyces cerevisiae