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Literature summary extracted from

  • Guddat, L.; Bardwell, J.; Martin, J.
    Crystal structures of reduced and oxidized DsbA Investigation of domain motion and thiolate stabilization (1998), Structure, 6, 757-767 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.15 expressed in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.15 the reduced enzyme form is crystallized by the dialysis method using 0.2 M ammonium acetate, 0.1 M sodium citrate pH 5.6, 30% (w/v) polyethylene glycol 4 K and 40 mM dithiothreitol. The oxidized enzyme form is crystallized by the hanging drop vapor diffusion method using 25% (w/v) polyethylene glycol 8 K in 0.1 M cacodylate buffer pH 6.5 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.15 a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues Escherichia coli
-
 a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.15 Escherichia coli P0AEG4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.15 Mono Q column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.15 a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues
-
 Escherichia coli a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.15 DsbA
-
 Escherichia coli