Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jirgis, E.N.; Bashiri, G.; Bulloch, E.M.; Johnston, J.M.; Baker, E.N.
    Structural views along the Mycobacterium tuberculosis MenD reaction pathway illuminate key aspects of thiamin diphosphate-dependent enzyme mechanisms (2016), Structure, 24, 1167-1177 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.9 expressed in Mycobacterium smegmatis strain mc24517 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.9 apoenzyme and in complex with thiamine diphosphate and Mg2+, sitting drop vapor diffusion method Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.9 Mg2+ required for activity, 1 mM used in assay conditions Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.9 isochorismate + 2-oxoglutarate Mycobacterium tuberculosis
-
 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?
2.2.1.9 isochorismate + 2-oxoglutarate Mycobacterium tuberculosis H37Rv
-
 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.9 Mycobacterium tuberculosis P9WK11
-
-
2.2.1.9 Mycobacterium tuberculosis H37Rv P9WK11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.9 IMAC column chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.9 isochorismate + 2-oxoglutarate
-
 Mycobacterium tuberculosis 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?
2.2.1.9 isochorismate + 2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.9 tetramer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.9 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase
-
 Mycobacterium tuberculosis
2.2.1.9 MenD
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.9 thiamine diphosphate dependent on Mycobacterium tuberculosis