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Literature summary extracted from

  • Jia, X.; Ye, X.; Chen, J.; Lin, X.; Vasseur, L.; You, M.
    Purification and biochemical characterization of a cyclodextrin glycosyltransferase from Geobacillus thermoglucosidans CHB1 (2018), Starch Staerke, 70, 1700016 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.19 EDTA about 110% activity at 10 mM Parageobacillus thermoglucosidasius

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 Al3+ less than 20% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Ba2+ about 55% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Cd2+ about 35% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Co2+ about 70% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Cu2+ about 20% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Fe2+ about 58% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Fe3+ about 28% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Hg2+ about 23% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 K+ about 85% residual activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.19 Mn2+ about 40% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Na+ about 90% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Pb2+ about 42% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 SDS complete inhibition at 1 mM Parageobacillus thermoglucosidasius
2.4.1.19 Zn2+ about 10% residual activity at 10 mM Parageobacillus thermoglucosidasius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Ca2+ about 110% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.19 Cd2+ about 110% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.19 Cu2+ about 108% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.19 Li+ about 118% activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Mg2+ about 130% activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.19 Mn2+ about 115% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.19 Ni2+ about 140% activity at 10 mM Parageobacillus thermoglucosidasius

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Parageobacillus thermoglucosidasius
-
-
-
2.4.1.19 Parageobacillus thermoglucosidasius CHB1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 maltodextrin + glycosyl acceptor
-
 Parageobacillus thermoglucosidasius alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 maltodextrin + glycosyl acceptor
-
 Parageobacillus thermoglucosidasius CHB1 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 potato starch + glycosyl acceptor
-
 Parageobacillus thermoglucosidasius alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 potato starch + glycosyl acceptor
-
 Parageobacillus thermoglucosidasius CHB1 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor 60.3% conversion rate with 3% (w/v) soluble starch Parageobacillus thermoglucosidasius alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor 60.3% conversion rate with 3% (w/v) soluble starch Parageobacillus thermoglucosidasius CHB1 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 70000, SDS-PAGE Parageobacillus thermoglucosidasius

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Parageobacillus thermoglucosidasius
2.4.1.19 cyclodextrin glycosyltransferase
-
 Parageobacillus thermoglucosidasius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 65
-
-
 Parageobacillus thermoglucosidasius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 50 80 the enzyme maintains more than 50% cyclization activity between 50 and 80°C Parageobacillus thermoglucosidasius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 50
-
 at incubation temperatures below 50°C, the enzyme maintains residual activity higher than 97% after 2 h. The residual activity of the enzyme decreases rapidly at temperatures higher than 50°C but remains stable at a lower level of activity Parageobacillus thermoglucosidasius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5.5
-
-
 Parageobacillus thermoglucosidasius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 5.5 9.5 after incubation at various pH values within a wide range (pH 5.5-9.5) for 2 h, the enzyme shows higher than 90% residual activity Parageobacillus thermoglucosidasius