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Literature summary extracted from

  • Marchetti, M.; Ronda, L.; Faggiano, S.; Liuzzi, A.; Percudani, R.; Bettati, S.
    Fluorescence quantification of allantoin in biological samples by cap-immobilized allantoinase/resorcinol assay (2018), Sens. Actuators B Chem., 255, 2820-2828 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.2.5 diagnostics the enzyme is useful for determination of allantoin in several human biological fluids, such as serum, urine, and saliva. Allantoin is a potential biomarker of oxidative stress in human physiology and pathology Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.5 recombinant enzyme expression Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.5 0.26
-
 (S)-allantoin pH 7.4, 37°C, recombinant enzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.5 (S)-allantoin + H2O Pseudomonas fluorescens
-
 allantoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.5 Pseudomonas fluorescens B5L363
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.5 recombinant enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.5 (S)-allantoin + H2O
-
 Pseudomonas fluorescens allantoate
-
 ?
3.5.2.5 additional information spectrometric fluorescence quantification of allantoin in biological samples by cap-immobilized allantoinase/resorcinol assay, formation of the lactone of 2,2',4,4'-tetrahydroxy-diphenylacetic acid at 100°C, specific for allantoin, method development and evaluation, overview. Other compounds such as sodium pyruvate, 2-oxoglutaric acid, 2-oxobutyric acid, oxalacetic acid, oxalic acid, formic acid, citric acid,glycolic acid, succinic acid, acetic acid, tartaric acid and salicylic acid do not form the fluorescent species. Also no reactivity with the intermediates of uricolytic pathway, 5-hydroxyisourate and 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline. The assay can be applied to the evaluation of the catalytic activity of PEGylated enzymes conceived for the treatment of hyperuricemia-related conditions Pseudomonas fluorescens ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.5.2.5 puuE
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.5 37
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.5 7.4
-
 assay at Pseudomonas fluorescens