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Literature summary extracted from

  • Shindou, H.; Shimizu, T.; Okinaga, T.; Seta, Y.; Tominaga, K.; Nishihara, T.
    Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells (2017), Sci. Rep., 7, 16701 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.23 additional information isozyme LPCAT4 is silenced in in ATDC5 cells, ATDC5 cells are transfected with LPCAT4 siRNA. LPCAT4 siRNA-transfected cells maintain viability 24 h after transfection. The transfected ATDC5 cells are cultured in alpha-MEM with ascorbic acid and ITS to induce chondrogenic differentiation. LPCAT4 siRNA transfection specifically suppresses mRNA expression of LPCAT4, without affecting LPCAT1-3 transcript levels, on day 15 after transfection. In control siRNA-transfected cells, LPCAT4 transcripts increase during chondrogenic differentiation. Knockdown of LPCAT4 does not change LPCAT enzymatic activity and the percentage of phosphatidylcholine species. Knockdown of LPCAT4 suppressed the mRNA expression of the chondrogenic differentiation markers, Col10, ALP, aggrecan, and TGF-beta and protein expression of Col10 on day 15 after transfection. The expression of Col2, Sox9, and Runx2 does not change. The knockdown of LPCAT4 suppresses the mRNA expression of BMP2, BMP6, and BMP7 during chondrogenic differentiation of ATDC5 cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine Mus musculus
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 CoA + 1,2-diacyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 additional information Mus musculus LPCAT4 has lysophosphatidylethanolamine acyltransferase as well as LPCAT activity. LPCAT uses lysophosphatidylcholine (LPC) as a substrate to generate phosphatidylcholine. Preference of LPCAT4 for oleoyl-CoA during chondrogenic differentiation. LPCAT4 does not prefer linoleoyl-, (5Z,8Z,11Z,14Z)-eicosatetraenoyl-, or docosahexaenoyl-CoA. Analysis of the fatty acid composition of whole cell lysates ?
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-
2.3.1.23 oleoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine Mus musculus
-
 CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Mus musculus Q6NVG1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 ATDC-5 cell
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 Mus musculus
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2.3.1.23 brain
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 Mus musculus
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2.3.1.23 C3H10T1/2 cell a pluripotent embryonic murine mesenchymal cell line Mus musculus
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2.3.1.23 cartilage LPCAT4 mRNA expression is stronger in the hypertrophic than in the prehypertrophic zone of cartilage Mus musculus
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2.3.1.23 chondrocyte
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 Mus musculus
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2.3.1.23 chondrogenic cell lysophosphatidylcholine acyltransferase 4 (LPCAT4) mRNA expression and LPCAT enzymatic activity towards oleoyl-, linoleoyl-, (5Z,8Z,11Z,14Z)-eicosatetraenoyl-, and docosahexaenoyl-CoA increases in the late stage of chondrogenic differentiation, when mineralization occurs Mus musculus
-
2.3.1.23 epididymis
-
 Mus musculus
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2.3.1.23 additional information LPCAT4 mRNA is highly expressed in the epididymis, brain, testis, and ovary Mus musculus
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2.3.1.23 ovary
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 Mus musculus
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2.3.1.23 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
-
 Mus musculus CoA + 1,2-diacyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 additional information LPCAT4 has lysophosphatidylethanolamine acyltransferase as well as LPCAT activity. LPCAT uses lysophosphatidylcholine (LPC) as a substrate to generate phosphatidylcholine. Preference of LPCAT4 for oleoyl-CoA during chondrogenic differentiation. LPCAT4 does not prefer linoleoyl-, (5Z,8Z,11Z,14Z)-eicosatetraenoyl-, or docosahexaenoyl-CoA. Analysis of the fatty acid composition of whole cell lysates Mus musculus ?
-
-
2.3.1.23 oleoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
-
 Mus musculus CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 LPCAT4
-
 Mus musculus
2.3.1.23 lysophosphatidylcholine acyltransferase 4
-
 Mus musculus

Expression

EC Number Organism Comment Expression
2.3.1.23 Mus musculus LPCAT4 mRNA expression increases during chondrogenic differentiation up

General Information

EC Number General Information Comment Organism
2.3.1.23 evolution LPLATs have been identified in the membrane-bound O-acyltransferase (MBOAT) and 1-acyl-glycerol-3-phosphate O-acyltransferase families. Lysophosphatidylcholine acyltransferases (LPCATs), including isozymes LPCAT1-4, have LPLAT activities other than LPCAT activity. For example, LPCAT4 has lysophosphatidylethanolamine acyltransferase as well as LPCAT activity Mus musculus
2.3.1.23 malfunction LPCAT4 knockdown decreases mRNA and protein levels of chondrogenic markers as well as Alcian blue staining intensity and alkaline phosphatase activity in ATDC5 cells. Knockdown of LPCAT4 suppresses the mRNA levels of chondrogenic differentiation markers, Col10, alkaline phosphatase (ALP), aggrecan, and transforming growth factor-beta (TGF-beta) and protein expression of Col10. LPCAT4 plays important roles during the transition of chondrocytes into hypertrophic chondrocytes and/or a mineralized phenotype. LPCAT4 knockdown inhibits hypertrophy/mineralization after a chondrogenic phenotype has been attained in ATDC5 cells Mus musculus
2.3.1.23 physiological function glycerophospholipids have important structural and functional roles in cells and are the main components of cellular membranes. Glycerophospholipids are formed via the de novo pathway (Kennedy pathway) and are subsequently matured in the remodeling pathway (Lands' cycle). Lands' cycle consists of two steps: deacylation of phospholipids by phospholipases A2 and reacylation of lysophospholipids by lysophospholipid acyltransferases (LPLATs). LPLATs play key roles in the maturation and maintenance of the fatty acid composition of biomembranes, and cell differentiation. Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells into chondrocytes. Lysophosphatidylcholine acyltransferase 4 (LPCAT4) mRNA expression and LPCAT enzymatic activity towards oleoyl-, linoleoyl-, (5Z,8Z,11Z,14Z)-eicosatetraenoyl-, and docosahexaenoyl-CoA increases in the late stage of chondrogenic differentiation, when mineralization occurs. Lysophosphatidylcholine (LPC) is involved in the pathogenesis of various lung disorders, including acute respiratory distress syndrom Mus musculus