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Literature summary extracted from
- Ylehd, an epoxide hydrolase with promiscuous haloalkane dehalogenase activity from tropical marine yeast Yarrowia lipolytica is induced upon xenobiotic stress (2017), Sci. Rep., 7, 11887 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.8.1.5 | 2-mercaptoethanol | 1-5 mM, activates | Yarrowia lipolytica |  |
| 3.8.1.5 | dithiothreitol | 1-5 mM, activates | Yarrowia lipolytica | |
| 3.8.1.5 | reduced glutathione | 1-5 mM, activates | Yarrowia lipolytica | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | degradation | potential biocatalyst for bioremediation/biosensing of mixed pollutants | Yarrowia lipolytica |
| 3.8.1.5 | environmental protection | potential biocatalyst for bioremediation/biosensing of mixed pollutants | Yarrowia lipolytica |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | the recombinant His-tagged enzyme is expressed in Escherichia coli BL21AI | Yarrowia lipolytica |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.8.1.5 | glycerol stabilizes the enzyme | Yarrowia lipolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.8.1.5 | Ag2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Co2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Cu2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Fe2+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Fe3+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Hg2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Mn2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Ni2+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Pb2+ | 1-5 mM, strong inhibition | Yarrowia lipolytica | |
| 3.8.1.5 | Zn2+ | 1-5 mM, complete inhibition | Yarrowia lipolytica | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | 1.24 | - |
1-bromododecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 3.8.1.5 | 4.15 | - |
1-bromodecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 3.8.1.5 | 5.12 | - |
1-Bromopropane | pH 4.5, 30°C | Yarrowia lipolytica | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.8.1.5 | cytosol | - |
Yarrowia lipolytica | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 43000 | - |
non-denaturing PAGE, SDS-PAGE | Yarrowia lipolytica |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | Glycerol | stabilizes the enzyme | Yarrowia lipolytica |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | Yarrowia lipolytica | Q6C598 | - |
- |
| 3.8.1.5 | Yarrowia lipolytica CLIB 122 | Q6C598 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | - |
Yarrowia lipolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 1-bromodecane + H2O | - |
Yarrowia lipolytica | 1-decanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromodecane + H2O | - |
Yarrowia lipolytica CLIB 122 | 1-decanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromododecane + H2O | - |
Yarrowia lipolytica | 1-dodecanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromododecane + H2O | - |
Yarrowia lipolytica CLIB 122 | 1-dodecanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | - |
Yarrowia lipolytica | 1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | - |
Yarrowia lipolytica CLIB 122 | 1-propanol + bromide | - |
? | |
| 3.8.1.5 | additional information | the bifunctioal enzyme also shows epoxidase activity | Yarrowia lipolytica | ? | - |
- |
|
| 3.8.1.5 | additional information | the bifunctioal enzyme also shows epoxidase activity | Yarrowia lipolytica CLIB 122 | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | monomer | 1 * 43000, SDS-PAGE | Yarrowia lipolytica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | HLD | - |
Yarrowia lipolytica |
| 3.8.1.5 | protein XP_504164 | - |
Yarrowia lipolytica |
| 3.8.1.5 | Ylehd | - |
Yarrowia lipolytica |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | 0.1 | - |
1-bromodecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 3.8.1.5 | 0.16 | - |
1-bromododecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 3.8.1.5 | 1.57 | - |
1-Bromopropane | pH 4.5, 30°C | Yarrowia lipolytica | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.8.1.5 | Yarrowia lipolytica | the expression of Ylehd is highly induced in presence of 1-bromodecane and 1,2-epoxyoctane but not in glucose grown cells. Higher expression in presence of xenobiotics | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | physiological function | xenobiotic stress mitigation | Yarrowia lipolytica |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | 2.12 | - |
1-Bromopropane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 3.8.1.5 | 8.8 | - |
1-bromododecane | pH 4.5, 30°C | Yarrowia lipolytica | |
| 3.8.1.5 | 10 | - |
1-bromodecane | pH 4.5, 30°C | Yarrowia lipolytica | |
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