EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.81 | expression in Escherichia coli | Enterobacter cloacae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.81 | 0.002 | - |
gentamicin C1 | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 0.003 | - |
gentamicin C2 | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 0.015 | - |
sisomicin | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 0.0802 | - |
kanamycin B | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 0.106 | - |
tobramycin | pH 7.6, 25°C | Enterobacter cloacae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.81 | Enterobacter cloacae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.81 | acetyl-CoA + gentamicin C1 | - |
Enterobacter cloacae | CoA + N3'-acetylgentamicin C1 | - |
? | |
2.3.1.81 | acetyl-CoA + gentamicin C2 | - |
Enterobacter cloacae | CoA + N3'-acetylgentamicin C2 | - |
? | |
2.3.1.81 | acetyl-CoA + kanamycin B | - |
Enterobacter cloacae | CoA + N3'-acetylkanamycin B | - |
? | |
2.3.1.81 | acetyl-CoA + sisomicin | - |
Enterobacter cloacae | CoA + N3'-acetyl-sisomicin | - |
? | |
2.3.1.81 | acetyl-CoA + tobramycin | - |
Enterobacter cloacae | CoA + N3'-acetyltobramycin | - |
? | |
2.3.1.81 | additional information | binding of aminoglycosides to AAC-VIa is enthalpically favored and entropically disfavored with a net result of favorable Gibbs energy. A net deprotonation of the enzyme, ligand, or both accompanies the formation of binary and ternary complexes. Poor substrates: kanamycn A, ribostamycin, neomycin B | Enterobacter cloacae | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.81 | More | AAC-VIa displays a monomer-dimer equilibrium in the absence of substrates.The dimer formation is concentration-dependent between 5 microM and 100 microM enzyme with more dimeric form appearing at higher concentrations. Binding of ligands shifts the enzyme to monomeric state | Enterobacter cloacae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.81 | AAC-VIa | - |
Enterobacter cloacae |
2.3.1.81 | aminoglycoside N3-acetyltransferase-VIa | - |
Enterobacter cloacae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.81 | 9.7 | - |
gentamicin C1 | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 14.5 | - |
kanamycin B | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 18.4 | - |
gentamicin C2 | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 24.5 | - |
sisomicin | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 43.5 | - |
tobramycin | pH 7.6, 25°C | Enterobacter cloacae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.81 | 200 | - |
kanamycin B | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 400 | - |
tobramycin | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 1600 | - |
sisomicin | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 4700 | - |
gentamicin C1 | pH 7.6, 25°C | Enterobacter cloacae | |
2.3.1.81 | 6100 | - |
gentamicin C2 | pH 7.6, 25°C | Enterobacter cloacae |