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Literature summary extracted from

  • Liu, J.; Kaganjo, J.; Zhang, W.; Zeilstra-Ryalls, J.
    Investigating the bifunctionality of cyclizing and classical 5-aminolevulinate synthases (2018), Protein Sci., 27, 402-410 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 expressed in Escherichia coli BL21 Gold (DE3) cells Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 T363S the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme Cereibacter sphaeroides
2.3.1.37 T83S the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme Cereibacter sphaeroides
2.3.1.37 T83S/T363S the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.0146
-
 succinyl-CoA wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.0774
-
 succinyl-CoA mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.1302
-
 succinyl-CoA mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.1645
-
 succinyl-CoA mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 11.3
-
 glycine mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 14
-
 glycine mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 16.7
-
 glycine mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 31.7
-
 glycine wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine Cereibacter sphaeroides
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 Ni superflow resin column chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 5-aminolevulinate-CoA weak cyclization activity Cereibacter sphaeroides 2-amino-3-hydroxycyclopent-2-en-1-one + CoASH
-
-
2.3.1.37 succinyl-CoA + glycine
-
 Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 ? x * around 50000, SDS-PAGE Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
2.3.1.37 ALA synthase
-
 Cereibacter sphaeroides
2.3.1.37 hemA
-
 Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 12
-
 glycine mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 12.1
-
 glycine mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 13.1
-
 glycine mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 63.4
-
 glycine wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate
-
 Cereibacter sphaeroides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.37 0.073
-
 succinyl-CoA mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.102
-
 succinyl-CoA mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.16
-
 succinyl-CoA mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.79
-
 glycine mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 0.87
-
 glycine mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 1.07
-
 glycine mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 2
-
 glycine wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides
2.3.1.37 4.34
-
 succinyl-CoA wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides