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Literature summary extracted from

  • Saida, F.; Odaert, B.; Uzan, M.; Bontems, F.
    First structural investigation of the restriction ribonuclease RegB NMR spectroscopic conditions, 13C/15N double-isotopic labelling and two-dimensional heteronuclear spectra (2004), Protein Expr. Purif., 34, 158-165 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.25 expression in Saccharomyces cerevisiae Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.25 H48A mutant enzyme binds its RNA substrate with the same affinity as the wild type protein. The beta-sheet content of the RegB H48A secondary structure is probably poor but not nil Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.25 a [pre-mRNA]-containing guanosine-adenosine + H2O Tequatrovirus T4
-
a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.25 Tequatrovirus T4 P13312
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.25
-
Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.25 a [pre-mRNA]-containing guanosine-adenosine + H2O
-
Tequatrovirus T4 a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
?
4.6.1.25 a [pre-mRNA]-containing guanosine-adenosine + H2O the enzyme cleaves with an almost absolute specificity its RNA substrate in the middle of the GGAG tetranucleotide mainly found in the Shine-Dalgarno sequence Tequatrovirus T4 a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.25 RegB
-
Tequatrovirus T4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.25 32
-
1.2 mM samples of RegB H48A precipitate at 32.5°C within 18 h. In the presence of sodium citrate molecules no visible precipitation after a 3 days-incubation Tequatrovirus T4