EC Number | Cloned (Comment) | Organism |
---|---|---|
4.6.1.25 | expression in Saccharomyces cerevisiae | Tequatrovirus T4 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.6.1.25 | H48A | mutant enzyme binds its RNA substrate with the same affinity as the wild type protein. The beta-sheet content of the RegB H48A secondary structure is probably poor but not nil | Tequatrovirus T4 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.25 | a [pre-mRNA]-containing guanosine-adenosine + H2O | Tequatrovirus T4 | - |
a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.6.1.25 | Tequatrovirus T4 | P13312 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.6.1.25 | - |
Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.25 | a [pre-mRNA]-containing guanosine-adenosine + H2O | - |
Tequatrovirus T4 | a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate | - |
? | |
4.6.1.25 | a [pre-mRNA]-containing guanosine-adenosine + H2O | the enzyme cleaves with an almost absolute specificity its RNA substrate in the middle of the GGAG tetranucleotide mainly found in the Shine-Dalgarno sequence | Tequatrovirus T4 | a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.6.1.25 | RegB | - |
Tequatrovirus T4 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.6.1.25 | 32 | - |
1.2 mM samples of RegB H48A precipitate at 32.5°C within 18 h. In the presence of sodium citrate molecules no visible precipitation after a 3 days-incubation | Tequatrovirus T4 |