EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.18 | expressed in Escherichia coli BL21(DE3) cells | Rhodothermus marinus |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.18 | the enzyme shows higher activity in sodium citrate buffer than sodium phosphate buffer at pH 6.0 and higher activity in sodium phosphate buffer than glycine buffer at pH 8.0 | Rhodothermus marinus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.18 | Ba2+ | 1 mM slightly reduces the enzyme activity (by approximately 10%) | Rhodothermus marinus | |
2.4.1.18 | Ca2+ | 1 mM slightly reduces the enzyme activity (by approximately 10%) | Rhodothermus marinus | |
2.4.1.18 | Cu2+ | 36.54% inhibition at 1 mM | Rhodothermus marinus | |
2.4.1.18 | EDTA | the enzyme retains 73.39%, 39.01%, 19.06%, 7.64%, and 3.30% of its activity in the presence of 1, 2, 4, 8, and 10 mM EDTA, respectively | Rhodothermus marinus | |
2.4.1.18 | Fe3+ | 17.33% inhibition at 1 mM | Rhodothermus marinus | |
2.4.1.18 | Mg2+ | 1 mM slightly reduces the enzyme activity (by approximately 10%) | Rhodothermus marinus | |
2.4.1.18 | Zn2+ | 1 mM slightly reduces the enzyme activity (by approximately 10%) | Rhodothermus marinus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.18 | Ca2+ | Ca2+ does not promote the activity of purified enzyme, but may be required for its activity | Rhodothermus marinus | |
2.4.1.18 | additional information | addition of Li+, Na+ or K+ has almost no effect on enzyme activity | Rhodothermus marinus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.18 | Rhodothermus marinus | - |
- |
- |
2.4.1.18 | Rhodothermus marinus STB05 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.18 | Ni-NTA resin chelating column chromatography | Rhodothermus marinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.18 | potato amylopectin type III | 100% activity | Rhodothermus marinus | potato amylopectin type III containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylopectin type III | 100% activity | Rhodothermus marinus STB05 | potato amylopectin type III containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylose | 65.4% activity compared to potato amylopectin type III | Rhodothermus marinus | potato amylose containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | potato amylose | 65.4% activity compared to potato amylopectin type III | Rhodothermus marinus STB05 | potato amylose containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | soluble starch | 47.3% activity compared to potato amylopectin type III | Rhodothermus marinus | soluble starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | soluble starch | 47.3% activity compared to potato amylopectin type III | Rhodothermus marinus STB05 | soluble starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | tapioca starch | 46.5% activity compared to potato amylopectin type III | Rhodothermus marinus | tapioca starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | tapioca starch | 46.5% activity compared to potato amylopectin type III | Rhodothermus marinus STB05 | tapioca starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | waxy corn starch | 74.7% activity compared to potato amylopectin type III | Rhodothermus marinus | waxy corn starch containing alpha-1,6-glucosidic linkages | - |
? | |
2.4.1.18 | waxy corn starch | 74.7% activity compared to potato amylopectin type III | Rhodothermus marinus STB05 | waxy corn starch containing alpha-1,6-glucosidic linkages | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.18 | ? | x * 73000, SDS-PAGE | Rhodothermus marinus |
2.4.1.18 | ? | x * 73200, calculated from amino acid sequence | Rhodothermus marinus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.18 | GBE | - |
Rhodothermus marinus |
2.4.1.18 | glycogen branching enzyme | - |
Rhodothermus marinus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 65 | - |
- |
Rhodothermus marinus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 50 | 80 | the enzyme has more than 60% activity between 50 and 80°C, 93.85% of maximal activity at 60°C, and 95.61% of maximal activity at 70°C | Rhodothermus marinus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 70 | 90 | the enzyme remains stable at temperatures up to 80°C and has a half-life at 85°C of approximately 31 min. The enzyme is quite stable (for at least 2 h) at 70°C or lower, and 92% of the initial enzyme activity is retained at 80°C for 2 h. Furthermore, the enzyme demonstrates half-lives at 85 and 90°C of 31 min and 2.5 min, respectively | Rhodothermus marinus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 7 | - |
- |
Rhodothermus marinus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 5.5 | 7.5 | the enzyme shows at least 80% of maximal activity from pH 5.5 to pH 7.5. The enzyme activity decreases rapidly at pH values lower than 5.5 and higher than 9.0 | Rhodothermus marinus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.18 | 3 | 11 | the enzyme has broad pH stability between pH 3.0 and 11.0 at 4°C, and prefers weakly acidic conditions at high temperatures (85°C). More than 96% of the initial enzyme activity is retained after incubation at pH 3.0-11.0 for 1 h. The enzyme loses all activity when incubated for 15 min at pH 3.0 or 4.0, and its half-life is 14.9 min at pH 5.0. The enzyme retains 72.1% of its initial activity after 1 h at pH 6.0 in sodium citrate buffer | Rhodothermus marinus |