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Literature summary extracted from

  • Zhang, J.; Li, M.; Zhang, Y.
    Enhancing the thermostability of recombinant cyclodextrin glucanotransferase via optimized stabilizer (2018), Process Biochem., 67, 64-70 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.19 chitosan the beta-CGTase and gamma-CGTase activities increase as the chitosan concentrations increases to 2% (w/v) Alkalihalobacillus pseudalcaliphilus
2.4.1.19 ethylene glycol with increasing concentrations of ethylene glycol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control Alkalihalobacillus pseudalcaliphilus
2.4.1.19 gelatin with increasing gelatin concentrations, both the beta-CGTase and gamma-CGTase activities show bell-shaped curves, with the highest increases of activities of 48.9% and 38.6%, respectively, at 1% (w/v) gelatin Alkalihalobacillus pseudalcaliphilus
2.4.1.19 glycerol with increasing concentrations of glycerol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control Alkalihalobacillus pseudalcaliphilus
2.4.1.19 mycose the beta-CGTase and gamma-CGTase activities increase as the mycose concentrations increases to 2% (w/v) Alkalihalobacillus pseudalcaliphilus
2.4.1.19 PEG400 the beta-CGTase and gamma-CGTase activities increase by 50.4% and 55.3% compared with that of control at the condition of 10% (w/v) PEG400 Alkalihalobacillus pseudalcaliphilus
2.4.1.19 Sodium acetate the highest increase in beta-CGTase activity is 46.9% at 1 mM sodium acetate which is 5.8fold higher than that of the control. The highest increase in gamma-CGTase activity is 39.1% at 2 mM sodium acetate which is 5.0fold higher than that of the control Alkalihalobacillus pseudalcaliphilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Komagataella phaffii Alkalihalobacillus pseudalcaliphilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Ca2+ the highest increase in beta-CGTase activity is 54.3% at 1 mM CaCl2 which is 5.6fold higher than that of the control. The highest increase in gamma-CGTase activity is 55.5% 1 mM CaCl2 which is 7.2fold higher than that of the control Alkalihalobacillus pseudalcaliphilus
2.4.1.19 Mg2+ the highest increase in beta-CGTase activity is 46.4% at 2 mM MgSO4 which is 4.8fold higher than that of the control. The highest increase in gamma-CGTase activity is 45.9% at 3 mM MgSO4 which is 5.9fold higher than that of the control Alkalihalobacillus pseudalcaliphilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Alkalihalobacillus pseudalcaliphilus
-
-
-
2.4.1.19 Alkalihalobacillus pseudalcaliphilus 8SB
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 potato starch + glycosyl acceptor
-
Alkalihalobacillus pseudalcaliphilus beta-cyclodextrin + gamma-cyclodextrin
-
?
2.4.1.19 potato starch + glycosyl acceptor
-
Alkalihalobacillus pseudalcaliphilus 8SB beta-cyclodextrin + gamma-cyclodextrin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 beta-CGTase
-
Alkalihalobacillus pseudalcaliphilus
2.4.1.19 CGTase
-
Alkalihalobacillus pseudalcaliphilus
2.4.1.19 cyclodextrin glucanotransferase
-
Alkalihalobacillus pseudalcaliphilus
2.4.1.19 gamma-CGTase
-
Alkalihalobacillus pseudalcaliphilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 50
-
-
Alkalihalobacillus pseudalcaliphilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 60 90 a chemical mixture (25% (v/v) glycerol, 10% (w/v) polyethylene glycol 400, and 0.5 mM CaCl2) is used as stabilizer to enhance the thermostability of the enzyme. The beta- and gamma-cyclodextrin glucanotransferase activities increase by 7.6- and 9.4-fold, respectively, compared with controls. The optimum temperature of enzyme activity increases from 50 to 60°C after the stabilizer application Alkalihalobacillus pseudalcaliphilus