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Literature summary extracted from

  • Goldstone, D.; Haebel, P.; Katzen, F.; Bader, M.; Bardwell, J.; Beckwith, J.; Metcalf, P.
    DsbC activation by the N-terminal domain of DsbD (2001), Proc. Natl. Acad. Sci. USA, 98, 9551-9556 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.16 expressed in Escherichia coli DH5alpha cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.16 C103A active site mutant Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.16 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.16 25000
-
 periplasmic domain DsbDalpha gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.16 a [protein] with reduced L-cysteine residues + thioredoxin disulfide Escherichia coli overall reaction a [protein] carrying a disulfide bond + thioredoxin
-
 ?
1.8.4.16 [DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide Escherichia coli
-
 [DsbC protein] carrying a disulfide bond + thioredoxin
-
 ?
1.8.4.16 [DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide Escherichia coli
-
 [DsbG protein] carrying a disulfide bond + thioredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.16 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.16 High Trap Ni-chelating column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.16 a [protein] with reduced L-cysteine residues + thioredoxin disulfide overall reaction Escherichia coli a [protein] carrying a disulfide bond + thioredoxin
-
 ?
1.8.4.16 [DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 Escherichia coli [DsbC protein] carrying a disulfide bond + thioredoxin
-
 ?
1.8.4.16 [DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 Escherichia coli [DsbG protein] carrying a disulfide bond + thioredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.16 ? x * 18000, periplasmic domain DsbDalpha, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.8.4.16 DsbD
-
 Escherichia coli
1.8.4.16 DsbDalpha N-terminal enzyme fragment, periplasmic domain Escherichia coli

General Information

EC Number General Information Comment Organism
1.8.4.16 metabolism the enzyme is required to maintain the functional oxidation state of DsbC and DsbG Escherichia coli