Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tikhonova, T.V.; Sorokin, D.Y.; Hagen, W.R.; Khrenova, M.G.; Muyzer, G.; Rakitina, T.V.; Shabalin, I.G.; Trofimov, A.A.; Tsallagov, S.I.; Popov, V.O.
    Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase (2020), Proc. Natl. Acad. Sci. USA, 117, 5280-5290 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.7 expressed in Escherichia coli BL21 Star (DE3) cells Thioalkalivibrio paradoxus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.2.7 hanging drop vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M sodium citrate, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol or 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% (w/v) PEG 8000, 15% (v/v) glycerol Thioalkalivibrio paradoxus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.2.7 E288A inactive Thioalkalivibrio paradoxus
1.8.2.7 H136A inactive Thioalkalivibrio paradoxus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.7 azide
-
 Thioalkalivibrio paradoxus
1.8.2.7 azide
-
 Thioalkalivibrio thiocyanoxidans
1.8.2.7 Cyanate mixed-type inhibition Thioalkalivibrio paradoxus
1.8.2.7 Cyanate mixed-type inhibition Thioalkalivibrio thiocyanoxidans
1.8.2.7 cyanide competitive inhibition Thioalkalivibrio paradoxus
1.8.2.7 cyanide competitive inhibition Thioalkalivibrio thiocyanoxidans
1.8.2.7 additional information nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM Thioalkalivibrio paradoxus
1.8.2.7 additional information nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM Thioalkalivibrio thiocyanoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.7 0.8
-
 Wurster's blue at pH 9.5 and 30°C Thioalkalivibrio paradoxus
1.8.2.7 1
-
 ferricytochrome c 550 at pH 9.5 and 30°C Thioalkalivibrio paradoxus
1.8.2.7 1.2
-
 potassium ferricyanide at pH 9.5 and 30°C Thioalkalivibrio paradoxus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.7 Cu2+ contains three copper ions Thioalkalivibrio paradoxus
1.8.2.7 Cu2+ contains three copper ions Thioalkalivibrio thiocyanoxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.7 Thioalkalivibrio paradoxus
-
-
-
1.8.2.7 Thioalkalivibrio paradoxus ARh1
-
-
-
1.8.2.7 Thioalkalivibrio thiocyanoxidans
-
-
-
1.8.2.7 Thioalkalivibrio thiocyanoxidans ARh2
-
-
-
1.8.2.7 Thioalkalivibrio thiocyanoxidans ARh4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.7 DEAE-Sepharose column chromatography and Superdex 200 gel filtration Thioalkalivibrio paradoxus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.7 additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio paradoxus ?
-
-
1.8.2.7 additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio thiocyanoxidans ?
-
-
1.8.2.7 additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio paradoxus ARh1 ?
-
-
1.8.2.7 additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio thiocyanoxidans ARh2 ?
-
-
1.8.2.7 additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio thiocyanoxidans ARh4 ?
-
-
1.8.2.7 thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio paradoxus cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio paradoxus ARh1 cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio paradoxus cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio paradoxus ARh1 cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
1.8.2.7 thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + reduced Wurster's blue + 2 H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + reduced Wurster's blue + 2 H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + reduced Wurster's blue + 2 H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio paradoxus cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio paradoxus ARh1 cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
1.8.2.7 thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + reduced Wurster's blue + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.7 homodimer 2 * 51891, calculated from amino acid sequence Thioalkalivibrio paradoxus
1.8.2.7 homodimer 2 * 51891, calculated from amino acid sequence Thioalkalivibrio thiocyanoxidans
1.8.2.7 homodimer 2 * 52000-53000, SDS-PAGE Thioalkalivibrio paradoxus
1.8.2.7 homodimer 2 * 52000-53000, SDS-PAGE Thioalkalivibrio thiocyanoxidans

Synonyms

EC Number Synonyms Comment Organism
1.8.2.7 TcDH
-
 Thioalkalivibrio paradoxus
1.8.2.7 TcDH
-
 Thioalkalivibrio thiocyanoxidans
1.8.2.7 thiocyanate dehydrogenase
-
 Thioalkalivibrio paradoxus
1.8.2.7 thiocyanate dehydrogenase
-
 Thioalkalivibrio thiocyanoxidans
1.8.2.7 thiocyanate: cytochrome c oxidoreductase
-
 Thioalkalivibrio paradoxus
1.8.2.7 thiocyanate: cytochrome c oxidoreductase
-
 Thioalkalivibrio thiocyanoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.7 9.5
-
-
 Thioalkalivibrio paradoxus
1.8.2.7 9.5
-
-
 Thioalkalivibrio thiocyanoxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.7 8 11 more than 50% activity between pH 8.0 and 11.0 with potassium ferricyanide as electron acceptor, more than 50% activity between pH 8.5 and 10.5 with potassium ferricyanide as electron acceptor Thioalkalivibrio paradoxus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.2.7 0.0014
-
 cyanide at pH 9.5 and 30°C Thioalkalivibrio paradoxus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.2.7 60
-
 at pH 9.5 and 30°C Thioalkalivibrio paradoxus azide