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Literature summary extracted from

  • Luo, Z.W.; Cho, J.S.; Lee, S.Y.
    Microbial production of methyl anthranilate, a grape flavor compound (2019), Proc. Natl. Acad. Sci. USA, 116, 10749-10756 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.277 gene aamt1, recombinant expresion in Escherichia coli strain W3110 trpD9923 and Corynebacterium glutamicum strain ATCC 13032 Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.277 additional information metabolic engineering of Escherichia coli and Corynebacterium glutamicum strains by expression of plant enzyme anthranilic acid methyltransferase1 (AAMT1). Optimizing the key enzyme anthranilic acid (ANT) methyltransferase1 (AAMT1) expression, increasing the direct precursor ANT supply, and enhancing the intracellular availability and salvage of the cofactor S-adenosyl-L-methionine required by AAMT1, results in improved methyl anthranilate (MANT) production in both engineered microorganisms. In situ two-phase extractive fermentation using tributyrin as an extractant is developed to overcome MANT toxicity. Fed-batch cultures of the final engineered Escherichia coli and Corynebacterium glutamicum strains in two-phase cultivation mode lead to the production of 4.47 and 5.74 g/l MANT, respectively, in minimal media containing glucose. Metabolic network related to MANT biosynthesis from glucose in Escherichia coli and Corynebacterium glutamicum, as well as metabolic engineering strategies, methods, overview Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.277 S-adenosyl-L-methionine + anthranilate Zea mays
-
 S-adenosyl-L-homocysteine + O-methyl anthranilate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.277 Zea mays D9J0Z7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.277 S-adenosyl-L-methionine + anthranilate
-
 Zea mays S-adenosyl-L-homocysteine + O-methyl anthranilate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.277 AAMT1
-
 Zea mays
2.1.1.277 ANT methyltransferase1
-
 Zea mays
2.1.1.277 anthranilic acid methyltransferase1
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.277 S-adenosyl-L-methionine
-
 Zea mays