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Literature summary extracted from

  • Dovala, D.; Rath, C.; Hu, Q.; Sawyer, W.; Shia, S.; Elling, R.; Knapp, M.; Metzger, L.I.
    Structure-guided enzymology of the lipid a acyltransferase LpxM reveals a dual activity mechanism (2016), Proc. Natl. Acad. Sci. USA, 113, E6064-E6071 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.243 heterologous overexpression in Escherichia coli membranes Acinetobacter baumannii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.243 crystals are grown by sitting-drop vapor diffusion at room temperature in 200 mM NaBr, 2.2 M (NH4)2SO4, at a protein:mother liquor ratio of 70:30 and an AbLpxM concentration of 21 mg/ml Acinetobacter baumannii

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.243 E127A mitation abrogates activity of the enzyme Acinetobacter baumannii
2.3.1.243 R159A mitation abrogates activity of the enzyme Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.243 0.001845
-
 dodecanoyl-[acyl-carrier protein] pH and temperature not specified in the publication Acinetobacter baumannii
2.3.1.243 0.009887
-
 octanoyl-[acyl-carrier protein] pH and temperature not specified in the publication Acinetobacter baumannii
2.3.1.243 0.01035
-
 tetradecanoyl-[acyl-carrier protein] pH and temperature not specified in the publication Acinetobacter baumannii
2.3.1.243 0.03047
-
 hexadecanoyl-[acyl-carrier protein] pH and temperature not specified in the publication Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.243 a tetradecanoyl-[acyl-carrier protein] + dodecanoyl-Kdo2-lipid IVA Acinetobacter baumannii
-
 dodecanoyl-(tetradecanoyl)-Kdo2-lipid IVA + an [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.243 Acinetobacter baumannii A0A1Y1P7X1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.243
-
 Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.243 a tetradecanoyl-[acyl-carrier protein] + dodecanoyl-Kdo2-lipid IVA
-
 Acinetobacter baumannii dodecanoyl-(tetradecanoyl)-Kdo2-lipid IVA + an [acyl-carrier protein]
-
 ?
2.3.1.243 dodecanoyl-[acyl-carrier protein] + dodecanoyl-Kdo2-lipid IVA the enzyme possesses acyltransferase and acylprotein thioesterase activities. The enzyme displays a strong preference for dodecanoyl-[acyl-carrier protein] over the other acyl chain lengths tested Acinetobacter baumannii dodecanoyl-(dodecanoyl)-Kdo2-lipid IVA + an [acyl-carrier protein]
-
 ?
2.3.1.243 hexadecanoyl-[acyl-carrier protein] + dodecanoyl-Kdo2-lipid IVA the enzyme possesses acyltransferase and acylprotein thioesterase activities. The enzyme displays a strong preference for dodecanoyl-[acyl-carrier protein] over the other acyl chain lengths tested Acinetobacter baumannii dodecanoyl-(hexadecanoyl)-Kdo2-lipid IVA + an [acyl-carrier protein]
-
 ?
2.3.1.243 octanoyl-[acyl-carrier protein] + dodecanoyl-Kdo2-lipid IVA the enzyme possesses acyltransferase and acylprotein thioesterase activities. The enzyme displays a strong preference for dodecanoyl-[acyl-carrier protein] over the other acyl chain lengths tested Acinetobacter baumannii dodecanoyl-(octanoyl)-Kdo2-lipid IVA + an [acyl-carrier protein]
-
 ?
2.3.1.243 tetradecanoyl-[acyl-carrier protein] + dodecanoyl-Kdo2-lipid IVA the enzyme possesses acyltransferase and acylprotein thioesterase activities. The enzyme displays a strong preference for dodecanoyl-[acyl-carrier protein] over the other acyl chain lengths tested Acinetobacter baumannii dodecanoyl-(tetradecanoyl)-Kdo2-lipid IVA + an [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.243 LpxM
-
 Acinetobacter baumannii

General Information

EC Number General Information Comment Organism
2.3.1.243 metabolism the enzyme is necessary for the biosynthesis of lipid A, which comprises the outer leaflet of the outer membrane in Gram-negative bacteria. The enzyme has important effects on virulence in many human and animal pathogen Acinetobacter baumannii