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Literature summary extracted from

  • Guo, J.; Zhou, Y.J.; Hillwig, M.L.; Shen, Y.; Yang, L.; Wang, Y.; Zhang, X.; Liu, W.; Peters, R.J.; Chen, X.; Zhao, Z.K.; Huang, L.
    CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts (2013), Proc. Natl. Acad. Sci. USA, 110, 12108-12113 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.175 synthesis establishment of miltiradiene production in Saccharomyces cerevisiae. Incorporation of CYP76AH1 and phyto-CYP reductase genes lead to heterologous production of ferruginol at 10.5 mg/l Salvia miltiorrhiza

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.175 expression in Saccharomyces cerevisiae Salvia miltiorrhiza

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.175 0.013
-
 abieta-8,11,13-triene pH 7.5, 28°C Salvia miltiorrhiza

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.175 microsome
-
 Salvia miltiorrhiza
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 Salvia miltiorrhiza
-
 abieta-8,11,13-trien-12-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.175 additional information Salvia miltiorrhiza CYP76AH1, catalyzes a four-electron oxidation cascade on miltiradiene to produce ferruginol both in vitro and in vivo ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.175 Salvia miltiorrhiza S4UX02
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.175 rhizome
-
 Salvia miltiorrhiza
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza abieta-8,11,13-trien-12-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.175 additional information CYP76AH1, catalyzes a four-electron oxidation cascade on miltiradiene to produce ferruginol both in vitro and in vivo Salvia miltiorrhiza ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.14.14.175 CYP76AH1
-
 Salvia miltiorrhiza

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.175 4.4
-
 abieta-8,11,13-triene pH 7.5, 28°C Salvia miltiorrhiza