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Literature summary extracted from

  • Thakur, N.; Kumar, V.; Thakur, S.; Sharma, N.; Sheeta, S.; Bhalla, T.
    Biotransformation of 4-hydroxyphenylacetonitrile to 4-hydroxyphenylacetic acid using whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 (2018), Proc. Biochem., 73, 117-123 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.5.5 synthesis the whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 is employed to develop a bioprocess for the synthesis of 4-hydroxyphenylacetic acid from 4-hydroxyphenylacetonitrile. A fed batch process designed at 500 ml with five feedings of substrate results in 150 mM product formation using 18 U/ml whole cells nitrilase (16 mg/ml dcw) at 45°C and a volumetric and catalytic productivity of 23 g/l and 4.13 g/g dcw/h Alcaligenes faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.5 4-hydroxyphenylacetic acid
-
 Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.5 20
-
 4-Hydroxyphenylacetonitrile pH 7.0, 30°C Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Alcaligenes faecalis
-
-
-
3.5.5.5 Alcaligenes faecalis MTCC 12629
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 4-aminoyphenylacetonitrile + H2O
-
 Alcaligenes faecalis ? + NH3
-
 ?
3.5.5.5 4-aminoyphenylacetonitrile + H2O
-
 Alcaligenes faecalis MTCC 12629 ? + NH3
-
 ?
3.5.5.5 4-hydroxyphenylacetonitrile + H2O
-
 Alcaligenes faecalis 4-hydroxyphenylacetic acid + NH3
-
 ?
3.5.5.5 4-hydroxyphenylacetonitrile + H2O
-
 Alcaligenes faecalis MTCC 12629 4-hydroxyphenylacetic acid + NH3
-
 ?
3.5.5.5 mandelonitrile + H2O
-
 Alcaligenes faecalis mandelic acid + NH3
-
 ?
3.5.5.5 mandelonitrile + H2O
-
 Alcaligenes faecalis MTCC 12629 mandelic acid + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Alcaligenes faecalis phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Alcaligenes faecalis MTCC 12629 phenylacetate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.5 40
-
 half-life: 45 h Alcaligenes faecalis
3.5.5.5 45
-
 half-life: 36 h Alcaligenes faecalis
3.5.5.5 50
-
 half-life: 13 h Alcaligenes faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.5.5 18
-
 4-hydroxyphenylacetic acid pH 7.0, 30°C Alcaligenes faecalis

Expression

EC Number Organism Comment Expression
3.5.5.5 Alcaligenes faecalis isobutyronitrile emerges as the most appropriate inducer with a 1.2-fold enhanced nitrilase production in inducer feeding approach up