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Literature summary extracted from

  • Brandt, C.; McFie, P.J.; Vu, H.; Chumala, P.; Katselis, G.S.; Stone, S.J.
    Identification of calnexin as a diacylglycerol acyltransferase-2 interacting protein (2019), PLoS ONE, 14, e0210396 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 calnexin calnexin is a diacylglycerol acyltransferase-2 interacting protein Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 recombinant expression of FLAG- or myc-tagged DGAT-2 fused to biotin ligase in HEK-293T cells and in COS-7 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information construction of chimeric enzyme BioId/DGAT2, DGAT2 is fused in-frame to the C-terminus of a promiscuous biotin ligase, BirA. Like DGAT2, BioId/DGAT2 stimulates the formation of large lipid droplets. Fusing DGAT2 to the C-terminus does not appear to change its localization or function in cells. When biotin is added to the culture medium, with or without oleate, there is a strong biotinylation signal that co-localizes with BioId/DGAT2 both in the endoplasmic reticulum and around lipid droplets. Protein interaction analysis of biotinylated BioId/DGAT2 in recombinant HEK-293T cells, overview Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 additional information no inhibition by selective DGAT1 inhibitor PF-04620110 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 endoplasmic reticulum membrane DGAT-2 is an integral membrane, it has two transmembrane domains separated by a short loop (about 5-8 amino acids) that extends into the endoplasmic reticulum (ER) lumen. The first transmembrane domain contains an ER targeting signal while the bulk of DGAT2 C-terminal to its second transmembrane is exposed to the cytosol Mus musculus 5789
-
2.3.1.20 lipid droplet
-
 Mus musculus 5811
-
2.3.1.20 microsome
-
 Mus musculus
-
-
2.3.1.20 additional information the bulk of DGAT2 C-terminal to its second transmembrane is exposed to the cytosol. Although present in the ER, DGAT2 also co-localizes with lipid droplets Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Mus musculus
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information Mus musculus calnexin is a DGAT2-interacting protein ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Mus musculus Q9DCV3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.20 additional information DGAT2 is degraded via the proteasome in a ubiquitin-dependent manner Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 recombinant biotinylated FLAG- or myc-tagged DGAT-2 fused to biotin ligase from HEK-293T cells, biotinylated proteins are captured with magnetic streptavidin beads Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 3T3-L1 cell
-
 Mus musculus
-
2.3.1.20 fibroblast
-
 Mus musculus
-
2.3.1.20 preadipocyte
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mus musculus CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information calnexin is a DGAT2-interacting protein Mus musculus ?
-
-
2.3.1.20 palmitoyl-CoA + 1,2-oleoyl-sn-glycerol usage of fluorescent NBD-tagged palmitoyl-CoA as substrate Mus musculus CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 More DGAT2 is part of a high molecular weight (about 650 kD) complex Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 acyl CoA:diacylglycerol acyltransferase-2
-
 Mus musculus
2.3.1.20 DGAT-2
-
 Mus musculus
2.3.1.20 DGAT2
-
 Mus musculus
2.3.1.20 diacylglycerol acyltransferase-2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.6
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.20 malfunction calnexin-deficient mouse embryonic fibroblasts have reduced intracellular triacylglycerol levels and fewer large lipid droplets Mus musculus
2.3.1.20 metabolism DGAT-2 atalyzes the final step of triacylglycerol (TG) biosynthesis Mus musculus
2.3.1.20 additional information DGAT2 is part of a high molecular weight (about 650 kD) complex. Calnexin, an endoplasmic reticulum chaperone, interacts with DGAT2. The interaction between calnexin and DGAT2 suggests that calnexin may play a role in lipid metabolism, including adipocyte differentiation. The subcellular localization and stability of DGAT2 are not altered by the absence of calnexin Mus musculus
2.3.1.20 physiological function DGAT2 catalyzes the formation of triacylglycerol (TG) using fatty acyl coenzyme A (CoA) and 1,2-diacylglycerol (DG) as substrates. TG is the major form of stored metabolic energy in eukaryotic organisms that is sequestered in the hydrophobic core of cytosolic lipid droplets until it is needed. DGAT-2 catalyzes TG synthesis for lipid droplet growth Mus musculus