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Literature summary extracted from

  • Harrus, D.; Khoder-Agha, F.; Peltoniemi, M.; Hassinen, A.; Ruddock, L.; Kellokumpu, S.; Glumoff, T.
    The dimeric structure of wild-type human glycosyltransferase B4GalT1 (2018), PLoS ONE, 13, e0205571 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.90 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.90 hanging drop vapor diffusion method, using 0.2 M potassium nitrate, 20% (w/v) PEG 3350 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.90 D315A the mutant shows about 90% of wild type activity Homo sapiens
2.4.1.90 H343A the mutant shows about 3% of wild type activity Homo sapiens
2.4.1.90 M340E the mutant shows about 85% of wild type activity Homo sapiens
2.4.1.90 M340H the mutant shows about 10% of wild type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.90 0.0455
-
 UDP-alpha-D-galactose at pH 6.3 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.90 Golgi apparatus
-
 Homo sapiens 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.90 Mn2+ 5 mM used in assay conditions Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.90 72600
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.90 UDP-alpha-D-galactose + N-acetyl-D-glucosamine Homo sapiens
-
 UDP + N-acetyllactosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.90 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.90 Bio-Scale mini profinity nickel cartridge chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.90 UDP-alpha-D-galactose + N-acetyl-D-glucosamine
-
 Homo sapiens UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDP-alpha-D-galactose + ovalbumin
-
 Homo sapiens UDP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.90 homodimer 2 * 36300, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.90 B4GALT1
-
 Homo sapiens
2.4.1.90 beta-1,4-galactosyltransferase 1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.90 0.059
-
 UDP-alpha-D-galactose at pH 6.3 and 37°C Homo sapiens