Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wani, T.A.; Pandith, S.A.; Gupta, A.P.; Chandra, S.; Sharma, N.; Lattoo, S.K.
    Molecular and functional characterization of two isoforms of chalcone synthase and their expression analysis in relation to flavonoid constituents in Grewia asiatica L. (2017), PLoS ONE, 12, e0179155 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expressed in Escherichia coli BL21(DE3) cells Grewia asiatica

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Grewia asiatica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 glutathione-Sepharose column chromatography Grewia asiatica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 flower
-
Grewia asiatica
-
2.3.1.74 leaf
-
Grewia asiatica
-
2.3.1.74 petal
-
Grewia asiatica
-
2.3.1.74 root highest expression of isoform CHS1 Grewia asiatica
-
2.3.1.74 stamen
-
Grewia asiatica
-
2.3.1.74 stem highest expression of isoform CHS2 Grewia asiatica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA the reaction has highest efficiency with isoform CHS1. 4-coumaroyl-CoA is the best substrate for isoform CHS1 Grewia asiatica 4 CoA + naringenin chalcone + 3 CO2
-
?
2.3.1.74 malonyl-CoA + acetyl-CoA the reaction has highest efficiency with isoform CHS2 Grewia asiatica ?
-
?
2.3.1.74 malonyl-CoA + butyryl-CoA the reaction has highest efficiency with isoform CHS2 Grewia asiatica ?
-
?
2.3.1.74 malonyl-CoA + hexanoyl-CoA the reaction has highest efficiency with isoform CHS2. Hexanoyl-CoA is the best substrate for isoform CHS2 Grewia asiatica ?
-
?
2.3.1.74 malonyl-CoA + octanoyl-CoA the reaction has highest efficiency with isoform CHS2 Grewia asiatica ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 homodimer 2 * 43000, SDS-PAGE Grewia asiatica

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 Chs1 isoform Grewia asiatica
2.3.1.74 Chs2 isoform Grewia asiatica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.74 Grewia asiatica isoform CHS2, calculated from amino acid sequence
-
7.6
2.3.1.74 Grewia asiatica isoform CHS1, calculated from amino acid sequence
-
10.1

Expression

EC Number Organism Comment Expression
2.3.1.74 Grewia asiatica the enzyme expression increases during flowering up