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Literature summary extracted from

  • Lazaro, B.; Villa, J.A.; Santin, O.; Cabezas, M.; Milagre, C.D.F.; de la Cruz, F.; Moncalian, G.
    Heterologous expression of a thermophilic diacylglycerol acyltransferase triggers triglyceride accumulation in Escherichia coli (2017), PLoS ONE, 12, e0176520 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.20 biotechnology WS/DGAT might have possible additional specificities, making it highly attractive for biotechnological applications such as biodiesel production Thermomonospora curvata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene Tcur_3818, DNA and amino acid sequence determination and analysis, recombinant expression of DGAT in Escherichia coli triggering rapid triglyceride accumulation in Escherichia coli. tDGAT is associated with the lipids accumulated in recombinant Escherichia coli cells Thermomonospora curvata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane
-
 Thermomonospora curvata 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Thermomonospora curvata
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Thermomonospora curvata DSM 43183
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Thermomonospora curvata D1AD40
-
-
2.3.1.20 Thermomonospora curvata DSM 43183 D1AD40
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 additional information strain DSM 43183 shows an optimal growing temperature of 50°C Thermomonospora curvata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thermomonospora curvata CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thermomonospora curvata DSM 43183 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information docking of palmitoyl-CoA into the donor pocket of the predicted tDGAT structure shows that the palmitoyl moiety is sandwiched between alpha5 helix and the beta sheet formed by beta9 and beta10 strands Thermomonospora curvata ?
-
-
2.3.1.20 additional information docking of palmitoyl-CoA into the donor pocket of the predicted tDGAT structure shows that the palmitoyl moiety is sandwiched between alpha5 helix and the beta sheet formed by beta9 and beta10 strands Thermomonospora curvata DSM 43183 ?
-
-
2.3.1.20 palmitoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thermomonospora curvata CoA + 1,2-diacyl-3-palmitoyl-sn-glycerol
-
 ?
2.3.1.20 palmitoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thermomonospora curvata DSM 43183 CoA + 1,2-diacyl-3-palmitoyl-sn-glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 51720, tDGAT, sequence calculation Thermomonospora curvata
2.3.1.20 More three-dimensional model of the tDGAT protein, structure homology modeling. tDGAT is predicted to have an acyl-CoA-dependent acyltransferase fold, with two domains connected by a helical linker. The core of the predicted N-terminal domain contains a four-stranded mixed sheet (beta2, beta5, beta6 and beta7) surrounded by three alpha-helices (alpha2, alpha4 and alpha5). The core of the predicted C-terminal domain consists of a five-stranded mixed sheet (beta8, beta9, beta10, beta11 and beta12) and five alpha-helices (alpha9, alpha10, alpha11, alpha13 and alpha14) covering the external face of the mixed sheet Thermomonospora curvata

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 ACY99349
-
 Thermomonospora curvata
2.3.1.20 DGAT
-
 Thermomonospora curvata
2.3.1.20 diacylglycerol acyltransferase
-
 Thermomonospora curvata
2.3.1.20 More cf. EC 2.3.1.75 Thermomonospora curvata
2.3.1.20 Tcur_3818
-
 Thermomonospora curvata
2.3.1.20 tDGAT
-
 Thermomonospora curvata
2.3.1.20 wax ester synthase/triacylglycerol:acylCoA acyltransferase
-
 Thermomonospora curvata
2.3.1.20 WS/DGAT
-
 Thermomonospora curvata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.20 Thermomonospora curvata sequence calculation
-
 6.44

Expression

EC Number Organism Comment Expression
2.3.1.20 Thermomonospora curvata successful introduction of the triacylglycerol biosynthesis pathway in gamma-proteobacteria, in an industrially appropriate microorganism, method development and optimization, overview. tDGAT is associated with the lipids accumulated in recombinant Escherichia coli cells. Optimization of fluorescence techniques for the detection of in situ TAG accumulation, Nile Red staining additional information

General Information

EC Number General Information Comment Organism
2.3.1.20 evolution Thermomonospora curvata acyltransferase ACY99349 belongs to the WS/DGAT family. tDGAT contains all the conserved motifs characteristic of WS/DGAT, mainly the catalytic site 140HHaavDG146, motif I 118PLW120, and motif II 281ND282. Like in other acyl-CoA-dependent acyltransferases the catalytic motif 140HHaavDG146, in the N-terminal domain of tDGAT, is predicted to be located in the hydrophobic pocket or channel that restricts the accessibility of hydrophilic substrates Thermomonospora curvata
2.3.1.20 additional information three-dimensional model of the tDGAT protein, structure homology modeling Thermomonospora curvata