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Literature summary extracted from

  • Lai, S.J.; Deng, Y.C.; Lai, M.C.
    Comparison of enzymatic traits between native and recombinant Glycine Sarcosine N-Methyltransferase from Methanohalophilus portucalensis FDF1T (2016), PLoS ONE, 11, e0168666 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.156 betaine feedback inhibition Methanohalophilus portucalensis
2.1.1.156 S-adenosyl-L-homocysteine competitive Methanohalophilus portucalensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.156 KCl increasing KCl concentration from 0.5 M to 1.5 M increases kcat/KM value almost 6fold Methanohalophilus portucalensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.156 34000
-
 and 56000, gel filtration Methanohalophilus portucalensis
2.1.1.156 56000
-
 and 34000, gel filtration Methanohalophilus portucalensis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.156 Methanohalophilus portucalensis F6KV61
-
-
2.1.1.156 Methanohalophilus portucalensis FDF-1 F6KV61
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.156 both native and recombinant protein Methanohalophilus portucalensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.156 164
-
 pH 7.3, 37°C Methanohalophilus portucalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.156 2 S-adenosyl-L-methionine + glycine
-
 Methanohalophilus portucalensis 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.156 2 S-adenosyl-L-methionine + glycine
-
 Methanohalophilus portucalensis FDF-1 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?