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Literature summary extracted from

  • Nissink, J.W.; Bista, M.; Breed, J.; Carter, N.; Embrey, K.; Read, J.; Winter-Holt, J.J.
    MTH1 substrate recognition - an example of specific promiscuity (2016), PLoS ONE, 11, e0151154 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.56 expressed in Escherichia coli Gold BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.56 enzyme in complex with substrates, hanging drop vapor diffusion method, using 25% (w/v) PEG3350 as precipitant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.56 2-(dimethylamino)-9-methyl-1,9-dihydro-6H-purin-6-one
-
 Homo sapiens
3.6.1.56 2-amino-9-methyl-1,9-dihydro-6H-purin-6-one
-
 Homo sapiens
3.6.1.56 2-amino-9-methyl-7,9-dihydro-1H-purine-6,8-dione
-
 Homo sapiens
3.6.1.56 3-[(2-amino-6-methylpyrimidin-4-yl)oxy]propan-1-ol
-
 Homo sapiens
3.6.1.56 6-(methylamino)pyrimidine-2,4(1H,3H)-dione
-
 Homo sapiens
3.6.1.56 9-methyl-2-(methylamino)-1,9-dihydro-6H-purin-6-one
-
 Homo sapiens
3.6.1.56 N-(1-methyl-1H-benzimidazol-5-yl)acetamide
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.56 0.0076
-
 8-oxo-dATP at pH 7.4, temperature not specified in the publication Homo sapiens
3.6.1.56 0.0113
-
 8-oxo-dGTP at pH 7.4, temperature not specified in the publication Homo sapiens
3.6.1.56 0.0134
-
 2-hydroxy-rATP at pH 7.4, temperature not specified in the publication Homo sapiens
3.6.1.56 0.014
-
 2-hydroxy-dATP at pH 7.4, temperature not specified in the publication Homo sapiens
3.6.1.56 0.082
-
 8-oxo-rGTP at pH 7.4, temperature not specified in the publication Homo sapiens
3.6.1.56 0.24
-
 8-oxo-rATP at pH 7.4, temperature not specified in the publication Homo sapiens
3.6.1.56 0.42
-
 dGTP at pH 7.4, temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.56 Homo sapiens P36639
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.56 Ni-NTA resin column chromatography and S75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.56 2-hydroxy-dATP + H2O
-
 Homo sapiens 2-hydroxy-dAMP + diphosphate
-
 ?
3.6.1.56 2-hydroxy-rATP + H2O
-
 Homo sapiens 2-hydroxy-rAMP + diphosphate
-
 ?
3.6.1.56 8-oxo-dATP + H2O
-
 Homo sapiens 8-oxo-dAMP + diphosphate
-
 ?
3.6.1.56 8-oxo-dGTP + H2O
-
 Homo sapiens 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.56 8-oxo-rATP + H2O
-
 Homo sapiens 8-oxo-rAMP + diphosphate
-
 ?
3.6.1.56 8-oxo-rGTP + H2O
-
 Homo sapiens 8-oxo-rGMP + diphosphate
-
 ?
3.6.1.56 dGTP + H2O
-
 Homo sapiens dGMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.56 MTH1
-
 Homo sapiens
3.6.1.56 NUDT1
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.56 0.0015
-
 pH and temperature not specified in the publication Homo sapiens 6-(methylamino)pyrimidine-2,4(1H,3H)-dione
3.6.1.56 0.0045
-
 pH and temperature not specified in the publication Homo sapiens 3-[(2-amino-6-methylpyrimidin-4-yl)oxy]propan-1-ol
3.6.1.56 0.059
-
 pH and temperature not specified in the publication Homo sapiens 9-methyl-2-(methylamino)-1,9-dihydro-6H-purin-6-one
3.6.1.56 0.08
-
 IC50 above 0.01 mM, pH and temperature not specified in the publication Homo sapiens N-(1-methyl-1H-benzimidazol-5-yl)acetamide
3.6.1.56 0.1
-
 IC50 above 0.1 mM, pH and temperature not specified in the publication Homo sapiens 2-(dimethylamino)-9-methyl-1,9-dihydro-6H-purin-6-one
3.6.1.56 0.1
-
 IC50 above 0.1 mM, pH and temperature not specified in the publication Homo sapiens 2-amino-9-methyl-1,9-dihydro-6H-purin-6-one
3.6.1.56 0.1
-
 IC50 above 0.1 mM, pH and temperature not specified in the publication Homo sapiens 2-amino-9-methyl-7,9-dihydro-1H-purine-6,8-dione