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Literature summary extracted from

  • Manat, G.; El Ghachi, M.; Auger, R.; Baouche, K.; Olatunji, S.; Kerff, F.; Touze, T.; Mengin-Lecreulx, D.; Bouhss, A.
    Membrane topology and biochemical characterization of the Escherichia coli BacA undecaprenyl-pyrophosphate phosphatase (2015), PLoS ONE, 10, e0142870 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.27 Lauryldimethylamine oxide the enzyme shows optimal activity at 0.1% (v/v) lauryldimethylamine oxide (4.3 mM) Escherichia coli
3.6.1.27 n-dodecyl-beta-D-maltopyranoside the enzyme activity appears optimal at low n-dodecyl-beta-D-maltopyranoside concentrations around 0.050-15% (v/v) (1-3 mM) Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.27 expressed in Escherichia coli C43(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.27 E21A the mutant shows 1.4% of wild type activity Escherichia coli
3.6.1.27 H30A the mutant shows 6.2% of wild type activity Escherichia coli
3.6.1.27 Q164A the mutant shows 24.3% of wild type activity Escherichia coli
3.6.1.27 R174A the mutant shows 0.13% of wild type activity Escherichia coli
3.6.1.27 R189A the mutant shows 12.1% of wild type activity Escherichia coli
3.6.1.27 S173A the mutant shows 2.2% of wild type activity Escherichia coli
3.6.1.27 S175A the mutant shows 41% of wild type activity Escherichia coli
3.6.1.27 S196A the mutant shows 36.8% of wild type activity Escherichia coli
3.6.1.27 S26A the mutant shows 15% of wild type activity Escherichia coli
3.6.1.27 S27A the mutant shows 0.013% of wild type activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.27 EDTA 95% inhibition at 0.05 mM, 98% inhibition at 1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.27 0.68
-
 undecaprenyl diphosphate at pH 7.5 and 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.27 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.27 Ca2+ the enzyme activity lost after treatment with 0.05 mM EDTA is not only fully recovered following addition of Ca2+ (1 mM) but also greatly enhanced by an about 9fold factor Escherichia coli
3.6.1.27 Mg2+ the enzyme activity lost after treatment with 0.05 mM EDTA is fully recovered following addition of Mg2+ (1 mM) Escherichia coli
3.6.1.27 Mn2+ the enzyme activity lost after treatment with 0.05 mM EDTA is partially recovered following addition of Mn2+ (1 mM) Escherichia coli
3.6.1.27 additional information no activity with Co2+, Fe2+, Zn2+, and Cu2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.27 undecaprenyl diphosphate + H2O Escherichia coli
-
 undecaprenyl phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.27 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.27 Ni-NTA column chromatography and Superdex S200 gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.27 5.5
-
 with diacylglycerol diphosphate as substrate, at pH 7.5 and 37°C Escherichia coli
3.6.1.27 10.3
-
 with farnesyl diphosphate as substrate, at pH 7.5 and 37°C Escherichia coli
3.6.1.27 11.3
-
 with undecaprenyl diphosphate as substrate, at pH 7.5 and 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.27 diacylglycerol diphosphate + H2O relatively good substrate Escherichia coli diacylglycerol phosphate + phosphate
-
 ?
3.6.1.27 farnesyl diphosphate + H2O
-
 Escherichia coli farnesyl phosphate + phosphate
-
 ?
3.6.1.27 additional information no activity with isopentenyl diphosphate, phosphatidylglycerol phosphate and phosphatidic acid Escherichia coli ?
-
-
3.6.1.27 undecaprenyl diphosphate + H2O
-
 Escherichia coli undecaprenyl phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.27 ? x * 25000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.6.1.27 bacA
-
 Escherichia coli
3.6.1.27 C55-PP phosphatase
-
 Escherichia coli
3.6.1.27 undecaprenyl-pyrophosphate phosphatase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.27 65 70 the enzyme has optimal activity at 65-70°C which is about 16 fold higher than at 37°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.27 73.5
-
 melting temperature Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.27 27
-
 undecaprenyl diphosphate at pH 7.5 and 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.27 5 7.5 a plateau of maximal activity is observed between 5.0 and 7.5. The enzyme does not show significant activity at acid pH values below 5.0 Escherichia coli