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Literature summary extracted from

  • Yang, S.U.; Kim, J.; Kim, H.; Suh, M.C.
    Functional characterization of Physcomitrella patens glycerol-3-phosphate acyltransferase 9 and an increase in seed oil content in Arabidopsis by its ectopic expression (2019), Plants (Basel), 8, 284-300 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.15 biotechnology PpGPAT9 may be another genetic resource to enhance storage oil yields from oilseed crops Physcomitrium patens
2.3.1.15 nutrition PpGPAT9 may be another genetic resource to enhance storage oil yields from oilseed crops Physcomitrium patens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 gene plat2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of eYFP-tagged PpGPAT9 in Nicotiana benthamiana leaf epidermal cell endoplasmic reticulum using Agrobacterium tumefaciens transfection system. Recombinant ectopic expression of PpGPAT9 in Arabidopsis thaliana increasing the seed oil content by approximately 10%. The levels of polyunsaturated fatty acids (18:2, 18:3, and 20:2) and saturated fatty acids (16:0, 18:0, and 20:0) increase by 60% and 43%, respectively, in the storage oil of the transgenic seeds of Arabidopsis thaliana when compared with the wild-type, the transgenic embryos with increased oil content contain larger embryonic cells than the wild-type. Quantitative RT-PCR expression analysis Physcomitrium patens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 endoplasmic reticulum membrane
-
 Physcomitrium patens 5789
-
2.3.1.15 protoplast
-
 Physcomitrium patens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Physcomitrium patens
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Physcomitrium patens A9SYM2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 archegonium
-
 Physcomitrium patens
-
2.3.1.15 gametophore
-
 Physcomitrium patens
-
2.3.1.15 microspore
-
 Physcomitrium patens
-
2.3.1.15 additional information the highest expression of PpGPAT9 is detected in the mature sporophyte (SM), followed by the spores. The PpGPAT9 transcript levels are approximately 3fold and 1.6fold higher in 4-week-old and 8-week-old gametophores than in the protonema Physcomitrium patens
-
2.3.1.15 protonema
-
 Physcomitrium patens
-
2.3.1.15 rhizoid
-
 Physcomitrium patens
-
2.3.1.15 sporophyte different stages Physcomitrium patens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Physcomitrium patens CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase 9
-
 Physcomitrium patens
2.3.1.15 GPAT9
-
 Physcomitrium patens
2.3.1.15 PHYPADRAFT_137573
-
 Physcomitrium patens
2.3.1.15 Ppa GPAT9
-
 Physcomitrium patens
2.3.1.15 PpGPAT9
-
 Physcomitrium patens

General Information

EC Number General Information Comment Organism
2.3.1.15 evolution GPAT9 is the homologue of a microsomal GPAT involved in the production of storage oil in mammalian cells Physcomitrium patens
2.3.1.15 malfunction loss-of-function mutant atgpat9 displays a lethal embryo phenotype, suggesting the essential role of GPAT9 in membrane lipid synthesis Physcomitrium patens
2.3.1.15 metabolism de novo fatty acid biosynthesis occurs exclusively in plastids, and glycerolipids are synthesized via prokaryotic pathway in the plastids and eukaryotic pathway in the endoplasmic reticulum. The synthesized fatty acyl-acyl carrier protein (ACP) or acyl-CoA is esterified to glycerol-3-phosphate by glycerol-3-phosphate acyltransferase (GPAT), overview. GPAT9 catalyzes the first step in the synthesis of membrane lipids and triacylglycerol (TAG) Physcomitrium patens
2.3.1.15 physiological function glycerol-3-phosphate acyltransferase 9 (GPAT9), which catalyzes the synthesis of lysophosphatidic acid (LPA) from a glycerol-3-phosphate and acyl-CoA, is isolated from the moss Physcomitrella patens, which produces high levels of very-long-chain polyunsaturated fatty acids (PUFAs) in protonema and gametophores Physcomitrium patens