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Literature summary extracted from

  • Klinska, S.; Jasieniecka-Gazarkiewicz, K.; Banas, A.
    Acyl-CoA lysophosphatidylcholine acyltransferases (LPCATs) of Camelina sativa seeds biochemical properties and function (2019), Planta, 250, 1655-1670 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.23 Ca2+ 27-62% inhibition at 0.05-2.0 mM Camelina sativa
2.3.1.23 Mg2+ 27-53% inhibition at 0.05-2.0 mM Camelina sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.23 microsome
-
 Camelina sativa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 alpha-linolenoyl-CoA + 1-oleoyl-lysophosphatidylcholine Camelina sativa
-
 CoA + 1-oleoyl-2-alpha-linolenoyl-lysophosphatidylcholine
-
 r
2.3.1.23 alpha-linolenoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine Camelina sativa
-
 CoA + 1-oleoyl-2-alpha-linolenoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 linoleoyl-CoA + 1-oleoyl-lysophosphatidylcholine Camelina sativa
-
 CoA + 1-oleoyl-2-linoleoyl-lysophosphatidylcholine
-
 r
2.3.1.23 linoleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine Camelina sativa
-
 CoA + 1-oleoyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 additional information Camelina sativa with the different seed microsomal fractions, substrate specificity, overview. Gondoyl-CoA and erucoyl-CoA, which are not accepted by CsLPCATs. The most preferred acyl donor in the assays with the microsomal fraction of the 24 and 31 days-after-flowering (DAF) seeds is linoleoyl-CoA, at 17 DAF alpha-linolenoyl-CoA is preferred. The activity of the backward reactions of the CsLPCATs in seed microsomal fractions is generally the highest at 17 DAF. Low activity with sn-2-O-glycero-3-phosphocholines (cf. EC 2.3.1.62) ?
-
-
2.3.1.23 oleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine Camelina sativa
-
 CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 oleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine Camelina sativa
-
 CoA + 1,2-dioleoyl-lysophosphatidylcholine
-
 r
2.3.1.23 palmitoyl-CoA + 1-oleoyl-lysophosphatidylcholine Camelina sativa low activity CoA + 1-oleoyl-2-palmitoyl-lysophosphatidylcholine
-
 r
2.3.1.23 stearoyl-CoA + 1-oleoyl-lysophosphatidylcholine Camelina sativa low activity CoA + 1-oleoyl-2-stearoyl-lysophosphatidylcholine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Camelina sativa
-
 cv. Suneson
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 embryo isolation of the microsomal fractions, three stages of seed development are chosen, 17, 24 and 31 days-after-flowering (DAF), in which it is possible to manually separate the embryo from the seed coat Camelina sativa
-
2.3.1.23 seed enzyme activity through seed development, overview Camelina sativa
-
2.3.1.23 silique
-
 Camelina sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 alpha-linolenoyl-CoA + 1-oleoyl-lysophosphatidylcholine
-
 Camelina sativa CoA + 1-oleoyl-2-alpha-linolenoyl-lysophosphatidylcholine
-
 r
2.3.1.23 alpha-linolenoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
-
 Camelina sativa CoA + 1-oleoyl-2-alpha-linolenoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 linoleoyl-CoA + 1-oleoyl-lysophosphatidylcholine
-
 Camelina sativa CoA + 1-oleoyl-2-linoleoyl-lysophosphatidylcholine
-
 r
2.3.1.23 linoleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
-
 Camelina sativa CoA + 1-oleoyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 additional information with the different seed microsomal fractions, substrate specificity, overview. Gondoyl-CoA and erucoyl-CoA, which are not accepted by CsLPCATs. The most preferred acyl donor in the assays with the microsomal fraction of the 24 and 31 days-after-flowering (DAF) seeds is linoleoyl-CoA, at 17 DAF alpha-linolenoyl-CoA is preferred. The activity of the backward reactions of the CsLPCATs in seed microsomal fractions is generally the highest at 17 DAF. Low activity with sn-2-O-glycero-3-phosphocholines (cf. EC 2.3.1.62) Camelina sativa ?
-
-
2.3.1.23 additional information 5 nmol of exogenous sn-1-oleoyl-lysophosphatidylcholine and 5 nmol of [14C]acyl-CoA are used as in vitro substrates, assay method optimization, overview Camelina sativa ?
-
-
2.3.1.23 oleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
-
 Camelina sativa CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
 r
2.3.1.23 oleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
-
 Camelina sativa CoA + 1,2-dioleoyl-lysophosphatidylcholine
-
 r
2.3.1.23 palmitoyl-CoA + 1-oleoyl-lysophosphatidylcholine low activity Camelina sativa CoA + 1-oleoyl-2-palmitoyl-lysophosphatidylcholine
-
 r
2.3.1.23 stearoyl-CoA + 1-oleoyl-lysophosphatidylcholine low activity Camelina sativa CoA + 1-oleoyl-2-stearoyl-lysophosphatidylcholine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 acyl-CoA:lysophosphatidylcholine acyltransferase
-
 Camelina sativa
2.3.1.23 CsLPCAT
-
 Camelina sativa
2.3.1.23 LPCAT
-
 Camelina sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 30
-
-
 Camelina sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.23 10 60 activity range, about 30% of maximal activity at 10°C, very low activity at 60°C, high activity at 20-40°C Camelina sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.23 30
-
 microsomal fractions, LPCAT activity is reduced by approximately 38% after 60 min, and 16% after 15 min Camelina sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.23 9
-
-
 Camelina sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.23 6 12 activity range, 64% of maximal activity at pH 11.0, 34% at pH 12.0, and 10% at pH 6.0 Camelina sativa

General Information

EC Number General Information Comment Organism
2.3.1.23 additional information lipid extraction and analysis from the seeds of Camelina sativa Camelina sativa