Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kageyama, H.; Tanaka, Y.; Takabe, T.
    Biosynthetic pathways of glycinebetaine in Thalassiosira pseudonana; functional characterization of enzyme catalyzing three-step methylation of glycine (2018), Plant Physiol. Biochem., 127, 248-255 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.162 expression in Escherichia coli Thalassiosira pseudonana

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.162 additional information GSDMT protein has two repeated AdoMet-binding motifs in the N-terminal and C-terminal domains. A truncation mutant carrying the N-terminal part exhibits high activity for glycine, low activity for sarcosine and no activity for dimethylglycine. A truncation mutant carrying the C-terminal part displays the highest activity to dimethylgycine, slightly lower to sarcosine and low activity to glycine Thalassiosira pseudonana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.162 0.73
-
 sarcosine wild-type, pH 8.0, 25°C Thalassiosira pseudonana
2.1.1.162 0.85
-
 glycine wild-type, pH 8.0, 25°C Thalassiosira pseudonana
2.1.1.162 1.1
-
 N,N-dimethylglycine wild-type, pH 8.0, 25°C Thalassiosira pseudonana
2.1.1.162 1.2
-
 N,N-dimethylglycine truncation mutant carrying the C-terminal part, pH 8.0, 25°C Thalassiosira pseudonana
2.1.1.162 1.5
-
 sarcosine truncation mutant carrying the C-terminal part, pH 8.0, 25°C Thalassiosira pseudonana
2.1.1.162 2
-
 glycine truncation mutant carrying the N-terminal part, pH 8.0, 25°C Thalassiosira pseudonana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.162 67000
-
 gel filtration Thalassiosira pseudonana

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.162 Thalassiosira pseudonana B8BQQ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.162 additional information no substrate: alanine, methionine Thalassiosira pseudonana ?
-
-
2.1.1.162 S-adenosyl-L-methionine + glycine
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + sarcosine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + betaine
-
-
2.1.1.162 S-adenosyl-L-methionine + sarcosine
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.162 monomer 1 * 65000, calculated from sequence Thalassiosira pseudonana

Synonyms

EC Number Synonyms Comment Organism
2.1.1.162 THAPSDRAFT_20797
-
 Thalassiosira pseudonana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.162 25
-
-
 Thalassiosira pseudonana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.162 8
-
 substrate dimethylglycine Thalassiosira pseudonana
2.1.1.162 9
-
 substrates glycine, sarcosine Thalassiosira pseudonana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.162 Thalassiosira pseudonana calculated from sequence
-
 4.9

Expression

EC Number Organism Comment Expression
2.1.1.162 Thalassiosira pseudonana expression is upregulated in high salinity up