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Literature summary extracted from

  • Giordano, D.; Provenzano, S.; Ferrandino, A.; Vitali, M.; Pagliarani, C.; Roman, F.; Cardinale, F.; Castellarin, S.D.; Schubert, A.
    Characterization of a multifunctional caffeoyl-CoA O-methyltransferase activated in grape berries upon drought stress (2016), Plant Physiol. Biochem., 101, 23-32 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 expressed in Escherichia coli BL21 cells Vitis vinifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.104 Ca2+
-
 Vitis vinifera
2.1.1.104 Mn2+
-
 Vitis vinifera
2.1.1.104 Zn2+
-
 Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.0827
-
 cyanidin 3-O-glucoside thrombin-cleaved enzyme, at pH 8.5 and 30°C Vitis vinifera
2.1.1.104 0.096
-
 caffeoyl-CoA GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera
2.1.1.104 0.11
-
 cyanidin 3-O-glucoside GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.104 Mg2+ 5 mM used in assay conditions Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Vitis vinifera Q43237 cultivar Barbera
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.104 glutathione Sepharose column chromatography Vitis vinifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.104 fruit
-
 Vitis vinifera
-
2.1.1.104 leaf
-
 Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Vitis vinifera S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + cyanidin 3-O-glucoside
-
 Vitis vinifera S-adenosyl-L-homocysteine + peonidin 3-O-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 CCoAOMT
-
 Vitis vinifera
2.1.1.104 COMT
-
 Vitis vinifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.104 0.0027
-
 cyanidin 3-O-glucoside GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera
2.1.1.104 0.0082
-
 caffeoyl-CoA GST-tagged recombinant enzyme, at pH 8.5 and 30°C Vitis vinifera
2.1.1.104 0.0129
-
 cyanidin 3-O-glucoside thrombin-cleaved enzyme, at pH 8.5 and 30°C Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.104 8.5
-
 optimal pH value for the methylation of cyanidin 3-O-glucoside Vitis vinifera

Expression

EC Number Organism Comment Expression
2.1.1.104 Vitis vinifera transcript accumulation of the enzyme is detected in berry skins, and increases during berry ripening on the plant, and in cultured berries treated with abscisic acid. Drought stress increases the enzyme transcript in berries up

General Information

EC Number General Information Comment Organism
2.1.1.104 physiological function the enzyme contributes to anthocyanin methylation activity in grape berries, in particular under drought stress Vitis vinifera