Literature summary extracted from

Wang, S.; Wang, R.; Liu, T.; Lv, C.; Liang, J.; Kang, C.; Zhou, L.; Guo, J.; Cui, G.; Zhang, Y.; Werck-Reichhart, D.; Guo, L.; Huang, L.
CYP76B74 catalyzes the 3''-hydroxylation of geranylhydroquinone in shikonin biosynthesis (2019), Plant Physiol., 179, 402-414 .

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.14.174	0.023	-	geranylhydroquinone	pH 7.5, 28°C	Arnebia euchroma

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.14.174	endoplasmic reticulum	-	Arnebia euchroma	5783	-
1.14.14.174	membrane	protein is anchored to the endoplasmic reticulum membrane	Arnebia euchroma	16020	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.14.14.174	geranylhydroquinone + [reduced NADPH-hemoprotein reductase] + O2	Arnebia euchroma	-	3''-hydroxygeranylhydroquinone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.174	Arnebia euchroma	A0A3Q9R4N5	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.14.14.174	leaf	-	Arnebia euchroma	-
1.14.14.174	root	hairy root	Arnebia euchroma	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.174	geranylhydroquinone + [reduced NADPH-hemoprotein reductase] + O2	-	Arnebia euchroma	3''-hydroxygeranylhydroquinone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.14.174	CYP76B74	-	Arnebia euchroma

General Information

EC Number	General Information	Comment	Organism
1.14.14.174	physiological function	In CYP76B74 RNA interference hairy roots, shikonin derivative accumulation decreases dramatically. CYP76B74 belongs to the CYP76B subfamily and catalyzes the key hydroxylation step in shikonin biosynthesis with high efficiency	Arnebia euchroma

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Release 2023.1 (January 2023)