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Literature summary extracted from

  • Iskandarov, U.; Silva, J.E.; Kim, H.J.; Andersson, M.; Cahoon, R.E.; Mockaitis, K.; Cahoon, E.B.
    A specialized diacylglycerol acyltransferase contributes to the extreme medium-chain fatty acid content of Cuphea seed oil (2017), Plant Physiol., 174, 97-109 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparison, RT-PCR expression analysis, recombinant expression in seeds of Camelina sativa. Microsomes of Camelina sativa seeds engineered for CpuDGAT1 expression display DGAT activity with 10:0-CoA and the diacylglycerol didecanoyl, that is approximately 4fold higher than that in camelina seed microsomes without CpuDGAT1, coexpression with a Cuphea viscosissima FatB thioesterase (CvFatB1) that generates 10:0 results in TAGs with nearly 15 mol% of 10:0. Recombinant expression of CpuDGAT1 and CvFatB1 with CvLPAT2, a 10:0-CoA-specific Cuphea LPAT, increases 10:0 amounts to 25 mol% in camelina seed TAG. These TAGs contain up to 40 mol% 10:0 in the sn-2 position, nearly double the amounts obtained from coexpression of CvFatB1 and CvLPAT2 alone. Although enriched in diacylglycerol, 10:0 is not detected in phosphatidylcholine in these seeds. Recombinant functional expression of CpuDGAT1 in the TAG-deficient Saccharomyces cerevisiae mutant strain H1246. CpuDGAT1 is able to rescue lipotoxicity of Saccharomyces cerevisiae H1246 cells grown in exogenous 0.25 mM C8:0 or C10:0, but not C18:1 Cuphea avigera

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information among the three DGAT2-like genes, only CpuDGAT2C functionally complements TAG biosynthesis in Saccharomyces cerevisiae mutant H1246 cells. Expression of CpuDGAT1 in the TAG-deficient Saccharomyces cerevisiae mutant strain H1246. CpuDGAT1 is able to rescue lipotoxicity of Saccharomyces cerevisiae H1246 cells grown in exogenous 0.25 mM C8:0 or C10:0, but not C18:1. Seed-specific recombinant CpuDGAT1 overexpression in Camelina sativa enhances C10:0 content in Camelina seeds. Coexpression of CpuDGAT1 and CvLPAT2 promotes accumulation of 10:0 at the TAG sn-2 position. Fatty acid composition of TAG and sn-2 position of TAG in mature seeds of wild-type camelina and camelina lines engineered for expression of CvFatB1 alone or with combinations of CpuDGAT1 and CvLPAT2, overview. CpuDGAT1 and CvLPAT2 coexpression increases 10:0 accumulation in DAG, but little 10:0 accumulation is detected in phosphatidylcholine (PC). Seed phenotype analysis Cuphea avigera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Cuphea avigera
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Cuphea avigera
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Cuphea avigera A0A193DVK9 var. pulcherrima
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 additional information no expression of seed-specific DGAT1 in root, stem, leaf, and flower Cuphea avigera
-
2.3.1.20 seed developing, seed-specific DGAT1 Cuphea avigera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Cuphea avigera CoA + 1,2,3-triacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 CpuDGAT1
-
 Cuphea avigera
2.3.1.20 DGAT1
-
 Cuphea avigera
2.3.1.20 DGAT1-type enzyme
-
 Cuphea avigera
2.3.1.20 diacylglycerol acyltransferase
-
 Cuphea avigera
2.3.1.20 seed-specific DGAT1
-
 Cuphea avigera
2.3.1.20 type 1 DGAT
-
 Cuphea avigera

General Information

EC Number General Information Comment Organism
2.3.1.20 metabolism seed-specific CpuDGAT1 is associated with medium-chain fatty acid metabolism Cuphea avigera
2.3.1.20 physiological function the specialized diacylglycerol acyltransferase DGAT1 contributes to the extreme medium-chain fatty acid content of Cuphea seed oil Cuphea avigera