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Literature summary extracted from

  • Roesler, K.; Shen, B.; Bermudez, E.; Li, C.; Hunt, J.; Damude, H.G.; Ripp, K.G.; Everard, J.D.; Booth, J.R.; Castaneda, L.; Feng, L.; Meyer, K.
    An improved variant of soybean type 1 diacylglycerol acyltransferase increases the oil content and decreases the soluble carbohydrate content of soybeans (2016), Plant Physiol., 171, 878-893 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli and Saccharomyces cerevisiae double null strain DELZTADGA1/DELTALRO1, recombinant expression of mutant CaDGAT1 in Glycine max Corylus americana
2.3.1.20 gene DGAT1, sequence comparisons, recombinant expression of wild-type and mutant enzymes, recombinant expression in Escherichia coli and Saccharomyces cerevisiae double null strain DELZTADGA1/DELTALRO1 Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information a type 1 DGAT complementary DNA (cDNA) from Corylus americana is isolated, CaDGAT1, improved variants from a screened library are created, then the corresponding amino acid substitutions are made in a Glycine max type 1 DGAT. Effects on soybean oil content and composition are determined following expression of the engineered GmDGAT1 in soybean somatic embryos of either the wild-type or the engineered variants of each DGAT, effect on soybean seed composition, detailed overview Glycine max
2.3.1.20 additional information the type 1 DGAT complementary DNA (cDNA) from Corylus americana is isolated, CaDGAT1, improved variants are created, library screening, and effect on Corylus americana seed composition, detailed overview. The corresponding amino acid substitutions are repeated in a Glycine max type 1 DGAT. Effects on soybean oil content and composition are determined following expression of the engineered GmDGAT1 in soybean somatic embryos of either the wild-type or the engineered variants of each DGAT, analysis of effect on soybean seed composition Corylus americana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane
-
 Glycine max 16020
-
2.3.1.20 membrane
-
 Corylus americana 16020
-
2.3.1.20 microsome
-
 Glycine max
-
-
2.3.1.20 microsome
-
 Corylus americana
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Glycine max
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Corylus americana
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Corylus americana A0A161IUT7
-
-
2.3.1.20 Glycine max I1MSF2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 seed developing Corylus americana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Glycine max CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Corylus americana CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-diacyl-sn-glycerol assay with radioactive oleoyl-CoA and DAG from microsome preparation of the recombinant yeast cells Glycine max CoA + 1,2-diacyl-3-oleoylglycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-diacyl-sn-glycerol assay with radioactive oleoyl-CoA and DAG from microsome preparation of the recombinant yeast cells Corylus americana CoA + 1,2-diacyl-3-oleoylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 61700, about, sequence calculation Corylus americana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 CaDGAT1
-
 Corylus americana
2.3.1.20 DGAT1
-
 Glycine max
2.3.1.20 DGAT1
-
 Corylus americana
2.3.1.20 GmDGAT1B
-
 Glycine max
2.3.1.20 type 1 diacylglycerol acyltransferase
-
 Glycine max
2.3.1.20 type 1 diacylglycerol acyltransferase
-
 Corylus americana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 25
-
 assay at Glycine max
2.3.1.20 25
-
 assay at Corylus americana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.2
-
 assay at Glycine max
2.3.1.20 7.2
-
 assay at Corylus americana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.20 Corylus americana sequence calculation
-
 8.93

General Information

EC Number General Information Comment Organism
2.3.1.20 additional information comparison of Glycine max and Corylus americana DGAT1 sequences Glycine max
2.3.1.20 additional information comparison of Glycine max and Corylus americana DGAT1 sequences Corylus americana