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Literature summary extracted from

  • Tran, H.; You, M.; Barbetti, M.
    Expression of defence-related genes in stems and leaves of resistant and susceptible field pea (Pisum sativum) during infection by Phoma koolunga (2018), Plant Pathol., 67, 156-166 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.270 gene hmm6, genotyping, gene expression analysis by quantitative real-time RT-PCR Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Pisum sativum
-
S-adenosyl-L-homocysteine + (+)-pisatin
-
?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Pisum sativum UWA H2012-81
-
S-adenosyl-L-homocysteine + (+)-pisatin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.270 Pisum sativum O24305
-
-
2.1.1.270 Pisum sativum UWA H2012-81 O24305
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.270 leaf high HMM6 enzyme expression level Pisum sativum
-
2.1.1.270 seedling
-
Pisum sativum
-
2.1.1.270 stem
-
Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
-
Pisum sativum S-adenosyl-L-homocysteine + (+)-pisatin
-
?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
-
Pisum sativum UWA H2012-81 S-adenosyl-L-homocysteine + (+)-pisatin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.270 (+)-6a-hydroxymaackiain 3-O-methyltransferase 1 UniProt Pisum sativum
2.1.1.270 6a-hydroxymaackiain methyltransferase
-
Pisum sativum
2.1.1.270 HMM1
-
Pisum sativum
2.1.1.270 hmm6
-
Pisum sativum
2.1.1.270 hydroxymaackiain methyltransferase
-
Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.270 S-adenosyl-L-methionine
-
Pisum sativum

General Information

EC Number General Information Comment Organism
2.1.1.270 metabolism the differential expression of 13 defence-related genes during Phoma koolunga infection of stems and leaves of susceptible versus resistant field pea (Pisum sativum) determined by quantitative RT-PCR shows that expression, in terms of relative mRNA level ratios, of genes encoding ferredoxin NADP oxidoreductase, 6a-hydroxymaackiain methyltransferase (hmm6), chalcone synthase (PSCHS3) and ascorbate peroxidase in leaves and stems vary during 6-72 hours post-inoculation (hpi) and reflecting host resistance levels in leaves versus stems. In comparison to the susceptible genotype, at 24, 48 and 72 hpi, two genes, hmm6 (122, 207 and 32fold, respectively) and PSCHS3 are strongly upregulated in leaves of the resistant genotype, highlighting that resistance against Phoma koolunga in field pea is governed by the early synthesis of pisatin, overview Pisum sativum
2.1.1.270 physiological function resistance against Phoma koolunga in field pea is governed by the early synthesis of pisatin involving the enzyme hydroxymaackiain methyltransferase Pisum sativum